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在tRNA甲基转移酶Trm1和RNA伴侣La之间的交叉影响真核tRNA成熟

Jennifer Porat¹, Ana Vakiloroayaei¹, Brittney M Remnant¹

¹Department of Biology, York University, Toronto, Canada.

The Journal of biological chemistry

|October 7, 2023

概括

裂变酵母Trm1,一个tRNA甲基转移酶,充当RNA陪伴者,促进tRNA折叠独立于其催化功能. 这种酶是这种酶.

科学领域:

分子生物学分子生物学
在RNA生物学,RNA生物学.
生物化学生物化学

背景情况:

转移RNA (tRNA) 需要广泛的转录后修改,以获得结构稳定性和适当的折叠.
在tRNA修改中的缺陷与人类疾病通过翻译受损,线粒体功能障碍和降解增加有关.
新出现的证据表明tRNA修饰酶可以充当伴侣,帮助tRNA折叠独立于它们的催化活性.

研究的目的:

为了研究裂变酵母tRNA甲基转移酶Trm1.1.的潜在RNA伴侣活性.
在tRNA成熟过程中探索Trm1与RNA伴侣La之间的功能相互作用.

主要方法:

在体内以tRNA为媒介的抑制试验被用来评估野生类型 (WT) 和催化死Trm1.1的功能.
在实验室中进行了RNA链回火和解离试验,以评估Trm1的伴侣类活动.
拉结合对Trm1介导的tRNA二甲基化的影响在体内和体外均进行了评估.

主要成果:

在tRNA介导的抑制试验中,WT和催化死Trm1都表现出活性,表明非催化功能.
在试验室中,Trm1表现出RNA链回火和解离活动,这是RNA伴侣体的特征.
发现La蛋白与新生tRNA前基因的结合会降低Trm1-介导的tRNA二甲基化.

结论:

裂变酵母Trm1具有RNA伴侣活性,在催化甲基化之外的tRNA成熟中发挥双重作用.
这些发现支持了tRNA修饰酶可以结合催化和非催化功能的假设.
拉蛋白结合影响Trm1的修饰活动,揭示了它们在tRNA处理中的协同作用的新方面.

关键词:

这是一个RNARNARNARNARNA.一个RNA伴侣.通过RNA甲基化.基因组RNA的修饰是RNA的修饰.这是一个tRNARNA.

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