在外围血液样本中的差异表达的circRNAs作为急性近角玻璃眼瘤的潜在生物标志物和治疗点
Ruijuan Guan1, Suonan Angxiu2, Ling Li3
1Ophthalmology Department, Qinghai Provincial People's Hospital, 2 Gonghe Road, Xining, 810000, Qinghai, China.
Scientific reports
|October 7, 2023
概括
循环RNAs (circRNAs) 参与了玻璃眼病的发生. 研究人员在青光眼患者中确定了345个差异表达的circRNAs (DEcircRNAs),表明它们可能是这种不可逆转失明的生物标志物.
科学领域:
- 眼科医生 眼科 眼科
- 分子生物学分子生物学
- 遗传学 遗传学 是一个
背景情况:
- 玻璃眼是全球不可逆转的失明的主要原因.
- 循环RNAs (circRNAs) 作为microRNA (miRNA) 海绵而起作用,并与各种疾病有关.
- 环RNAs在玻璃眼病原体中的作用尚不清楚.
研究的目的:
- 为了识别涉及初级角关闭光眼 (PACG) 的circRNAs.
- 调查差异表达的circRNAs (DEcircRNAs) 在青光眼病原发生中的潜在作用.
- 探索DEcircRNAs作为潜在的生物标志物和治疗眼的治疗点.
主要方法:
- 来自PACG患者的外周血液样本的转录组测序.
- 生物信息学分析以识别DEcircRNA及其目标基因.
- 定量逆转录PCR (qRT-PCR) 用于验证特定的circRNA-基因相互作用.
主要成果:
- 345个DEcircRNA和481个差异表达的基因在青光眼患者中被确定.
- 11 预计DEcircRNAs将调节五个基因作为miRNA海绵.
- hsa-circ-0000745表达与潜在的目标基因NEAT1表达正相关.
结论:
- DEcircRNAs参与了一个与免疫细胞功能和青光眼进展相关的调节网络.
- 周围血液中的circRNAs显示为绿眼病的诊断生物标志物具有前途.
- DEcircRNAs代表了治疗眼的潜在治疗点.
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