CTNNBIP1-CLSTN1 作为一个管家的仿制RNA,并通过 SERPINE2 调节细胞增殖
Chen Chen1,2, Fujun Qin1,3, Sandeep Singh4,5
1School of Basic Medical Sciences, Academy of Medical Sciences, Zhengzhou University, Zhengzhou, 450001, Henan, China.
Cell death discovery
|October 7, 2023
概括
化学RNA曾经被认为是癌症特异性,现在被认为是正常细胞中必不可少的"家政化学RNA". CTNNBIP1-CLSTN1融合转录在调节细胞增殖和生存方面发挥着至关重要的作用.
科学领域:
- 分子生物学分子生物学
- 基因组学就是基因组学.
- 细胞生物学 细胞生物学
背景情况:
- 化学RNAs传统上与癌症和染色体重组有关.
- 在正常生理学中,关于仿制RNAs功能的实验证据有限.
研究的目的:
- 在正常生理背景下研究仿制RNACTNNBIP1-CLSTN1的表达和功能.
- 挑战对仿制RNAs作为癌症特异性的传统理解.
主要方法:
- 在各种组织和细胞类型 (癌症和非癌症) 中对CTNNBIP1-CLSTN1的定量表达分析.
- 功能性研究涉及静止CTNNBIP1-CLSTN1在非癌细胞中,以评估对细胞增殖,细胞循环和细胞亡的影响.
- 救援实验与奇默和野生类型基因的外源表达.
- 调查涉及SERPINE2的下游监管途径.
主要成果:
- CTNNBIP1-CLSTN1在许多正常组织和细胞类型中得到广泛表达.
- 在癌症和非癌症细胞系之间,CTNNBIP1-CLSTN1的表达水平没有显著差异.
- 沉默CTNNBIP1-CLSTN1导致细胞增殖减少,G2/M细胞周期停止,以及多种细胞类型的细胞亡.
- 嵌合体的外源表达,但不是野生类型的基因,恢复了细胞循环停止,证实了嵌合体的作用.
- 发现CTNNBIP1-CLSTN1通过SERPINE2调节细胞增殖.
结论:
- CTNNBIP1-CLSTN1代表了一种新的类型的
- 家庭管理 仿真RNAs
- 对于正常的细胞功能至关重要.
- 这一发现重新定义了超越瘤过程的化学RNAs的作用.
- CTNNBIP1-CLSTN1是细胞增殖和正常细胞生存的关键调节者.
相关概念视频
02:23Abnormal Proliferation
4.6K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.6K
01:23Negative Regulator Molecules
35.4K
Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
35.4K
01:45Regulation of Nuclear Protein Sorting
2.4K
Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...
2.4K
01:58Cell Specific Gene Expression
13.6K
Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
13.6K
01:16TGF - β Signaling Pathway
7.4K
The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...
7.4K
03:03mTOR Signaling and Cancer Progression
3.8K
The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
The mTOR pathway or the...
3.8K