在干旱压力期间,在红杉中对WRKY转录因子家族进行全基因组识别和全面分析

相关实验视频

Updated: Jul 14, 2025

Phosphoproteomic Strategy for Profiling Osmotic Stress Signaling in Arabidopsis

Phosphoproteomic Strategy for Profiling Osmotic Stress Signaling in Arabidopsis

Published on: June 25, 2020

Xianming Song^1,2, Xianfei Hou¹, Youling Zeng³

¹Economic Crop Research Institute, Xinjiang Academy of Agricultural Sciences, Urumqi, 830091, China.

Scientific reports

|October 7, 2023

概括

研究人员在花中发现了82个WRKY基因,揭示了它们在植物发育和干旱应激反应中的作用. CtWRKY55显示出作为提高红杉干旱耐受性的关键基因的显著潜力.

科学领域:

植物分子生物学植物分子生物学
基因组学就是基因组学.
转录因子转录因子

背景情况:

转录因子的WRKY家族对于植物发育和应激反应至关重要.
关于红杉 (Carthamus tinctorius L.) 中的WRKY基因家族的研究有限.

研究的目的:

为了识别和表征红杉中的WRKY基因家族.
为了研究CtWRKY基因在红杉对干旱压力的反应中的作用.

主要方法:

对CtWRKY基因的全基因组识别.
对CtWRKY基因的遗传学和结构分析.
对基因结构,保存域,动机,染色体定位和重复事件的分析.
促使者cis作用元素分析.
在干旱压力下对基因表达进行转录组和定量实时PCR (qRT-PCR) 分析.

主要成果:

总共有82个CtWRKY基因被确定并分为3个主要组和5个子组.
在子家族中观察到保存的结构和图案.
CtWRKY基因分布在12个染色体中,碎片重复影响了它们的进化.
促进剂含有对非生物压力和荷尔蒙反应的元素.
CtWRKY基因表现出组织特异性表达,并对干旱压力做出强烈反应.
在干旱和补水期间,CtWRKY55的表达增加了八倍以上,这表明它发挥了关键作用.

结论:

这项研究提供了对红杉中CtWRKY基因家族的全面分析.
CtWRKY基因参与了红杉对干旱压力的反应.
CtWRKY55是一种潜在的关键基因,可以通过分子繁殖来增强耐旱性.

更多相关视频

Measuring Gene Expression in Bombarded Barley Aleurone Layers with Increased Throughput

Measuring Gene Expression in Bombarded Barley Aleurone Layers with Increased Throughput

Published on: March 30, 2018

Author Spotlight: Polysome Profiling Protocol for Studying Translational Regulation in Arabidopsis Under Heat Stress

Author Spotlight: Polysome Profiling Protocol for Studying Translational Regulation in Arabidopsis Under Heat Stress

Published on: October 11, 2024

相关概念视频

Responses to Drought and Flooding

Responses to Drought and Flooding

Water plays a significant role in the life cycle of plants. However, insufficient or excess of water can be detrimental and pose a serious threat to plants.

Adaptations that Reduce Water Loss

Adaptations that Reduce Water Loss

Though evaporation from plant leaves drives transpiration, it also results in loss of water. Because water is critical for photosynthetic reactions and other cellular processes, evolutionary pressures on plants in different environments have driven the acquisition of adaptations that reduce water loss.

Responses to Salt Stress

Responses to Salt Stress

Salt stress—which can be triggered by high salt concentrations in a plant’s environment—can significantly affect plant growth and crop production by influencing photosynthesis and the absorption of water and nutrients.