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Next-generation Sequencing

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....

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Updated: Jul 14, 2025

Detection of Rare Genomic Variants from Pooled Sequencing Using SPLINTER

Detection of Rare Genomic Variants from Pooled Sequencing Using SPLINTER

Published on: June 23, 2012

通过阿尔戈纳特介导的晶体管平台实现超快单核酸变异检测.

Derong Kong^1,2, Shen Zhang^1,2, Mingquan Guo³

¹State Key Laboratory of Molecular Engineering of Polymers, Department of Macromolecular Science, Fudan University, Shanghai, 200433, P. R. China.

Advanced materials (Deerfield Beach, Fla.)

|October 8, 2023

概括

此摘要是机器生成的。

快速单核酸变异 (SNV) 检测现在可以使用一种新的石墨烯场效应晶体管 (GFET) 平台. 这项技术实现了对各种核酸点的高灵敏度和速度,而无需放大.

关键词:

阿尔戈纳特蛋白质是一种蛋白质.生物传感器,场效应晶体管.医疗服务点检测试验单核酸变异的变化

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Last Updated: Jul 14, 2025

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Published on: June 23, 2012

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科学领域:

生物技术是生物技术.
纳米技术纳米技术
分子生物学分子生物学

背景情况:

目前的单核酸变异 (SNV) 检测方法通常需要漫长的程序,如核酸放大或测序,限制了快速诊断.
现有的SNV检测技术的灵敏度和速度之间存在显著的权衡,特别是对于稀少样品.

研究的目的:

开发一种快速,灵敏和特定的方法,用于"测试和运行"SNV检测.
克服传统SNV检测的局限性,消除了样本准备步骤的需要,如核酸提取,反转录和放大.

主要方法:

开发一个与阿尔戈诺特蛋白集成的石墨烯场效应晶体管 (GFET) 平台.
利用Argonaute蛋白质创建一个纳米级的结合通道,用于预先组织DNA探针,从而加速目标结合.
使用GFET-Argonaute系统在未放大核酸样本中直接检测SNV,包括microRNA,循环瘤DNA和cDNA.

主要成果:

开发的GFET平台可以通过单核酸分辨率检测SNV.
该系统成功地识别了各种核酸点的种子区域中的SNV,包括瘤相关的microRNA,循环瘤DNA,病毒RNA和逆转录cDNA.
一个集成的微芯片在5分钟内同时检测到多个SNV,取得了与测序相似的结果.

结论:

阿尔戈纳特介绍的GFET平台为快速和敏感的SNV检测提供了一种开创性的"测试即用"解决方案.
这种方法显著减少了检测时间和复杂性,为更快的 Point-of-care 诊断铺平了道路.
该技术在没有先前核酸操纵的情况下,在各种生物样本中检测SNV的广泛适用性.