相关实验视频

Updated: Jul 14, 2025

Deacetylation Assays to Unravel the Interplay between Sirtuins SIRT2 and Specific Protein-substrates

Deacetylation Assays to Unravel the Interplay between Sirtuins SIRT2 and Specific Protein-substrates

Published on: February 27, 2016

SIRT1 Asn346 糖链促进原脱乙烯化,在压力下对骨质细胞具有保护作用

Min Cai¹, Yaoqi Chen², Yiting Lin²

¹Department of Geriatric Medicine, Fujian Provincial Hospital, Shengli Clinical Medical College, Fujian Medical University, Fuzhou, Fujian, China.

Biochemical and biophysical research communications

|October 8, 2023

概括

对SIRT1的O-GlcNAc修饰增强了其脱乙酶活性,通过RANK/RANKL通路保护骨质细胞. 这一发现揭示了SIRT1的存在.

科学领域:

生物化学生物化学
细胞生物学细胞生物学
骨生物学骨生物学骨生物学

背景情况:

赛尔图因1 (SIRT1) 是一种依赖NAD+的脱乙酶,对于骨重塑和衰老等生理过程至关重要.
骨质细胞衰老和骨重塑受SIRT1活动的影响.
需要阐明O-GlcNAc修饰在SIRT1在压力下骨质母细胞中的功能中的作用.

研究的目的:

调查核SIRT1的O-GlcNAc修饰是否会在压力下增强其脱乙酶活性.
为了确定这种修改是否通过RANK/RANKL信号通路保护骨质母细胞.
探索SIRT1在原脱乙和骨重塑中的作用.

主要方法:

使用R和在线数据进行生物信息分析,以确定SIRT1在骨代谢中的作用.
对涉及SIRT1.1的途径进行丰富分析.
免疫涂抹和免疫光检测以确认蛋白质的定位.
质谱测量用于识别O-糖化位.
用于蛋白质结构分析的PyMol.
用RT-PCR进行基因转录分析.

主要成果:

SIRT1与骨质细胞转录,亡和脱乙烯化途径有关.
SIRT1和O-糖化催化酶 (OGT) 定位在骨质细胞核中.
在SIRT1的中心域中,在阿斯巴拉金346 (N346) 发生O-糖化,这是参与OGT结合的区域.
在压力下,O-糖基化SIRT1表现出增加的脱乙酶活性.
RANKL的SIRT1脱甲基化调节骨质细胞存活率和亡,通过RANK/RANKL通路影响骨重塑.

结论:

在压力下,O-GlcNAc的修饰增强了SIRT1脱乙酶在骨质细胞中的活性.
修改后的SIRT1通过脱乙RANKL来保护骨质母细胞,从而调节RANK/RANKL信号通路.
在压力下骨重塑过程中,SIRT1在维持骨质细胞存活率和亡之间的平衡中发挥着重要作用.

关键词:

骨的新陈代谢骨的新陈代谢脱乙化脱乙化脱乙化骨质母细胞的骨质细胞.骨质疏松症是一种骨质疏松症.一个SIRT1系统.

更多相关视频

Skeletal Phenotype Analysis of a Conditional Stat3 Deletion Mouse Model

Skeletal Phenotype Analysis of a Conditional Stat3 Deletion Mouse Model

Published on: July 3, 2020

Using Real-Time Cell Metabolic Flux Analyzer to Monitor Osteoblast Bioenergetics

Using Real-Time Cell Metabolic Flux Analyzer to Monitor Osteoblast Bioenergetics

Published on: March 1, 2022

相关概念视频

Osteoclasts in Bone Remodeling

Osteoclasts in Bone Remodeling

Osteoclasts are cells responsible for bone resorption and remodeling. They originate from hematopoietic progenitor cells present in the bone marrow. Numerous progenitor cells fuse to form multinucleated cells, each with 10-20 nuclei. A single osteoclast has a diameter of 150 to 200 µM. These cells have ruffled borders that break down the underlying bone tissue and release minerals such as calcium into the blood in bone resorption. Osteoclasts cling to bones with their ruffled edges during...

Role of ER in the Secretory Pathway

Role of ER in the Secretory Pathway

Eukaryotic cells have a special pathway that enables communication between various intracellular membrane-bound compartments and also with the extracellular environment. This pathway is termed as the secretory pathway.
Components of the secretory pathway
About a third of proteins synthesized in the cell are sorted via the secretory route. They shuffle between different compartments in membrane-bound vesicles until they reach their final destination. The main intracellular compartments involved...

TGF - β Signaling Pathway

TGF - β Signaling Pathway

The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...

Type IV Collagen of Basal Lamina

Type IV Collagen of Basal Lamina

Type IV collagen is a 400 nm long, network-forming collagen that acts as a barrier between the epithelial and endothelial cells. Type IV collagen forms the backbone of the basement membrane by scaffolding with laminin, entactin, proteoglycans, and fibronectin. Apart from rendering structural support to the basement membrane, it also helps entail signaling potentials necessary for both pathological and physiological functions.
A type IV collagen molecule has six alpha chains which can...

Structural Protein Function

Structural Protein Function

Structural proteins are a category of proteins responsible for functions ranging from cell shape and movement to providing support to major structures such as bones, cartilage, hair, and muscles. This group includes proteins such as collagen, actin, myosin, and keratin.
Collagen, the most abundant protein in mammals, is found throughout the body. In connective tissue, such as skin, ligaments, and tendons, it provides tensile strength and elasticity. In bones and teeth, it mineralizes to...

The Effect of Aging on Tissues

The Effect of Aging on Tissues

Several body functions deteriorate with age. The external signs of aging are easily identifiable. For example, the skin becomes dry, less elastic, and thins out, forming wrinkles. The skin of the face begins to appear looser due to a decrease in the levels of elastic and collagen fibers in the connective tissue. Additionally, melanin production in the hair follicle decreases with age, resulting in gray hair. Moreover, the senses of sight and hearing decline, so glasses and hearing aids may...