相关实验视频

Updated: Jul 14, 2025

Chromatin Isolation by RNA Purification ChIRP

Chromatin Isolation by RNA Purification ChIRP

Published on: March 25, 2012

核长非编码RNA的功能领域:对基因调节和细胞内架构的洞察

Chikashi Obuse¹, Tetsuro Hirose²

¹Graduate School of Science, Osaka University, Toyonaka 560-0043, Japan; Institute for Open and Transdisciplinary Research Initiatives (OTRI), Osaka University, Suita 565-0871, Japan.

Current opinion in cell biology

|October 8, 2023

概括

长非编码RNAs (lncRNAs) 调节基因表达和细胞结构. 它们独特的RNA结构可以被小分子准,提供了一种新的治疗策略.

科学领域:

分子生物学分子生物学
在RNA生物学,RNA生物学.
药物发现药物发现药物发现

背景情况:

长非编码RNAs (lncRNAs) 是基因表达和细胞组织的关键调节者.
像Xist和NEAT1_2这样的关键lncRNA在X染色体无活化和斑形成等过程中发挥着重要作用.
这些lncRNA具有模块化结构,可以与特定的RNA结合蛋白 (RBPs) 结合,从而定义它们的功能.

研究的目的:

研究针对 lncRNA 内的RNA 结构进行治疗干预的潜力.
探索 lncRNAs作为一种新型的治疗点.

主要方法:

对lncRNA结构及其与RNA结合蛋白 (RBPs) 的相互作用的功能分析.
在 lncRNA 中识别由 RBP 识别的特征性 RNA 结构.
设计用于通过破坏RBP-RNA相互作用来调节lncRNA功能的小型化学化合物的概念化.

主要成果:

lncRNAs具有不同的结构域,可以作为特定RBP的平台.
在lncRNAs中的特定RNA结构被RBPs识别,决定lncRNA的功能.
这些与RBP结合的RNA结构易受小化学化合物的调制.

结论:

嵌入在lncRNAs中的复杂RNA结构代表了一个重要的,以前未被开发的治疗点类.
准这些RNA结构为调节lncRNA功能和开发新疗法提供了一个有希望的策略.

关键词:

染色体是什么染色体是什么染色体是什么异性染色是一种异性染色.没有膜的有机细胞.阶段分离阶段分离阶段分离结构 RNA 结构 RNA 结构小型化合物小型化合物在ncRNA中,我们可以

更多相关视频

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA

Published on: April 10, 2018

Detection of RNA-binding Proteins by In Vitro RNA Pull-down in Adipocyte Culture

Detection of RNA-binding Proteins by In Vitro RNA Pull-down in Adipocyte Culture

Published on: July 22, 2016

相关概念视频

lncRNA - Long Non-coding RNAs

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...

Additional Subnuclear Structures

Additional Subnuclear Structures

The eukaryotic nucleus is a double membrane-bound organelle that contains nearly all of the cell’s genetic material in the form of chromosomes. It is rightly called the “brain” of the cell as it shoulders the responsibility of responding to various physiological processes, stress, altered metabolic conditions, and other cellular signals.
The nucleus contains many membrane-less subnuclear organelles or nuclear bodies, such as nucleoli, Cajal bodies, speckles,...

Regulation of Nuclear Protein Sorting

Regulation of Nuclear Protein Sorting

Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...

Chromatin Position Affects Gene Expression

Chromatin Position Affects Gene Expression

Chromatin is the massive complex of DNA and proteins packaged inside the nucleus. The complexity of chromatin folding and how it is packaged inside the nucleus greatly influences access to genetic information. Generally, the nucleus' periphery is considered transcriptionally repressive, while the cell's interior is considered a transcriptionally active area.
Topologically Associated Domains (TADs)
The 3-dimensional positioning of chromatin in the nucleus influences the...

Types of RNA

Types of RNA

Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...

The Nucleus

The Nucleus

The nucleus is a membrane-bound organelle that acts as a control center in a eukaryotic cell. It contains chromosomal DNA, which controls gene expression and precisely regulates the production of proteins within the cell. In contrast, the DNA inside the mitochondria and chloroplast only carries out functions that are specific to those organelles.
Arrangement of DNA within Nucleus
The regulation of gene expression inside the nucleus is dependent on many factors, including the DNA structure. The...