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经验诱导的海马后突触蛋白质组和蛋白质组的重塑

Seok Heo¹, Taewook Kang², Alexei M Bygrave¹

¹Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA; Kavli Neuroscience Discovery Institute, Johns Hopkins University, Baltimore, Maryland, USA.

Molecular & cellular proteomics : MCP

|October 8, 2023

概括

经验重塑了大脑的形状.

科学领域:

神经科学是一个神经科学.
分子生物学分子生物学
蛋白质组学是指蛋白质组学.

背景情况:

突触后密度 (PSD) 对突触可塑性至关重要.
了解体验诱导的PSD变化是突触重塑的关键.

研究的目的:

在不同的经验后调查海马PSD蛋白质和蛋白质组的全球动态.
揭示经验依赖的突触重塑的分子机制.

主要方法:

开发了基于iTRAQ的修改后的TiSH协议,用于特定站点的蛋白质组分析.
分析了从被训练在抑制性回避 (IA) 任务中的小鼠身上获得的海马PSD部分.
使用质谱学识别和量化PSD蛋白和蛋白.

主要成果:

在PSD中确定了3938种蛋白质和2761种蛋白质.
检测到6188种独特的酸盐,许多IA调节的蛋白显示酸化降低.
生物信息分析揭示了谷氨酸受体功能的调节,RHO GTPase循环和突触可塑性.
识别了IA调节的激酶和酶,包括调节AMPA受体表面表达的Ppm1h.

结论:

经验动态重塑PSD蛋白质组和蛋白质组.
这些发现为了解突触可塑性和大脑中经验依赖的变化提供了资源.

关键词:

抑制性回避是一种抑制性回避.学习和记忆的学习和记忆.光蛋白质组学光蛋白质组学光蛋白质组学后突触密度后突触密度

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