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单胺在回收水中的冠状病毒无活化的有效性
Aidan R Foster1, Charles N Haas2, Charles P Gerba1
1Water & Energy Sustainable Technology (WEST) Center, University of Arizona, Tucson, AZ, USA.
The Science of the total environment
|October 8, 2023
概括
单胺在废水中有效消毒人类冠状病毒229E. 水质没有显著影响单胺.
科学领域:
- 环境微生物学 环境微生物学
- 水处理技术水处理技术
- 病毒学 病毒学
背景情况:
- 污水处理厂 (WWTP) 对于减少传染性病毒负载至关重要.
- 传统消毒剂自由因消毒副产品而面临监管审查.
- 化剂在WWTP中越来越多地被采用,需要研究其对抗SARS-CoV-2等病毒的有效性.
研究的目的:
- 评估单胺对人类冠状病毒229E (HCoV-229E) 的消毒功效.
- 为了比较单胺在酸盐缓冲盐水 (PBS) 和回收废水中的有效性.
- 为了确定HCoV-229E.的单胺消毒所需的Ct99.9值.
主要方法:
- 人类冠状病毒229E (HCoV-229E) 暴露于PBS和回收废水中预先形成的单胺 (3 mg/L).
- 在六个月内收集回收水,并补充自由氨 (1毫克/升) 来模仿WWTP条件.
- 在MRC-5宿主细胞单层上使用TCID50试验评估病毒活力.
主要成果:
- 对于HCoV-229E.的单胺消毒,平均Ct99.9的176 mg*min/L被确定为HCoV-229E.
- 在单胺处理的回收水和PBS之间没有观察到无活化率的显著差异.
- 回收废水的水矩阵并没有显著影响单胺的消毒效率.
结论:
- 单胺是废水中冠状病毒的强效消毒剂.
- 单胺对HCoV-229E的有效性不受水矩阵的显著影响.
- 这些发现支持单胺在废水处理中用于病毒失活的使用.
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