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朱古伊万通过PI3K/AKT信号通路促进骨质生成:网络药理学分析和实验验证
Shuo Yang1, Bin Zhang1, Yu-Guo Wang2
1Department of Stomatology, The First Medical Center, Chinese PLA General Hospital, Beijing, 100853, China.
Current medical science
|October 8, 2023
概括
祖古伊万 (Zuo Gui Wan,简称ZGW) 通过激活PI3K/AKT通路,促进干细胞中的骨形成. 这种传统中医药增强了骨质生成标志物,并验证了其对骨缺陷的治疗潜力.
科学领域:
- 药理学和中国传统医学 (TCM)
- 细胞生物学和再生医学
- 生物化学和分子生物学
背景情况:
- 骨质生成对于骨修复至关重要,它是TCM处方Zuo Gui Wan (ZGW) 的目标.
- ZGW促进骨质生成的精确分子机制在很大程度上仍未被阐明.
- 网络药理学提供了一个系统层面的方法来发现复杂的药物-草药相互作用.
研究的目的:
- 为了研究Zuo Gui Wan (ZGW) 促进骨质生成的潜在分子机制.
- 通过使用网络药理学,确定由ZGW调制的关键目标和途径.
- 实验验证ZGW在骨髓介质干细胞 (BMSCs) 的骨质分化中的作用.
主要方法:
- 网络药理学分析以确定与骨质生成相关的ZGW目标和途径.
- 生物信息分析包括目标基因查,蛋白-蛋白相互作用 (PPI) 网络构建,基因本体学 (GO) 和KEGG通路丰富.
- 使用BMSC进行实验验证,以评估性酸酶 (ALP) 活性,基因和蛋白质表达 (ALP,OCN,Runx 2) 以及PI3K/AKT通路的西部斑分析.
主要成果:
- 网络药理学确定了487个ZGW生物活性成分标,其中175个与骨质生成标重叠,以及28个核心标.
- GO和KEGG分析强调了细胞反应和关键信号通路的参与,特别是PI3K/AKT通路.
- 实验验证证证实,ZGW增强了骨质生成标记物 (ALP,OCN,Runx2) 并显著激活PI3K/AKT通路,PI3K抑制剂的作用是可逆的.
结论:
- 祖古伊万 (Zuo Gui Wan,简称ZGW) 有效地促进骨髓中介质干细胞 (BMSCs) 的骨质生成.
- ZGW的亲骨质效应是通过激活酸-3-酶/蛋白酶B (PI3K/AKT) 信号通路进行的.
- 这项研究为ZGW在骨修复和再生医学中的传统使用提供了机制基础.
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