克罗辛通过调节PIM1/FOXO3a轴来改善下肢深静脉血栓症
Jun Chen1, Yi Yang2, Shuo Wang3
1Department of Vascular Surgery, Suqian Hospital Affiliated to Xuzhou Medical University, (Nanjing Drum Tower Hospital Group Suqian Hospital), Suqian 223800, Jiangsu, China. laojucheng19960813@163.com.
Cellular and molecular biology (Noisy-le-Grand, France)
|October 9, 2023
概括
沙夫兰中发现的一种化合物克罗辛在治疗下肢深静脉血栓症 (LEDVT) 中表现有前途. 它保护内皮细胞免受损伤,减少细胞死亡,促进血管形成,表明潜在的治疗作用.
科学领域:
- 药理学 药理学是指药理学的学科.
- 细胞生物学 细胞生物学
- 血管生物学 血管生物学
背景情况:
- 下肢深静脉血栓塞 (LEDVT) 由于高发病率和死亡率而带来重大健康挑战.
- 克罗辛是一种天然化合物,具有潜在的抗凝固和抗血栓性质.
- 克罗辛可能影响LEDVT的具体机制仍然不完全理解.
研究的目的:
- 在LEDVT的背景下研究素的治疗潜力.
- 阐明克罗辛对内皮细胞的影响背后的细胞和分子机制.
- 确定PIM1/Foxo3a信号通路在crocin的作用中的作用.
主要方法:
- 人类静脉内皮细胞 (HUVEC) 用血栓素和不同度的crocin进行治疗.
- 细胞活力,细胞亡和血管生成使用MTT试验,流细胞计,TUNEL试验和管形成试验进行了评估.
- 分子对接,RT-qPCR,双化酶记者测定和RIP用于分析PIM1/Foxo3a轴.
主要成果:
- 克罗辛在剂量取决的方式显著提高了用血栓治疗的HUVECs的活力.
- 克罗辛抑制了血栓激素诱导的亡,并在HUVEC中促进了血管生成.
- 确定了PIM1作为crocin的直接标,在LEDVT和血栓治疗细胞上调;其调节影响了细胞行为,Foxo3a与PIM1.1相互作用.
结论:
- 克罗辛显示出对由血栓激发的内皮细胞损伤的保护作用,减少细胞亡并增强血管生成.
- 克罗辛的治疗作用通过PIM1/Foxo3a信号通路进行介导.
- 这些发现表明,克罗辛具有作为LEDVT治疗剂的巨大潜力.
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