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基于UHPLC-MS/MS的中央碳代谢揭示了与结肠癌相关的生物标志物

Weisi Yuan¹, Yinxing Chen², Dan Zhuang³

¹Department of Colorectal and Anal Surgery, Chaozhou Central Hospital Affiliated to Southern Medical University, Chaozhou, Guangdong, 521000, China. 13829011177@163.com.

Cellular and molecular biology (Noisy-le-Grand, France)

|October 9, 2023

概括

研究人员确定了结肠癌中的关键代谢变化,发现腺单酸盐 (AMP),脱氧胺单酸盐 (dTMP),果糖和D-葡萄糖显示出作为早期癌症检测的新生物标志物的巨大潜力.

科学领域:

在瘤学瘤学.
代谢学代谢学代谢学
生物化学生物化学

背景情况:

结肠癌是癌症死亡的主要原因之一.
早期检测显著提高了生存率.
改变的代谢物水平正在被研究为潜在的癌症生物标志物.

研究的目的:

为了确定用于结肠癌检测的代谢生物标志物.
分析癌症与准癌症组织中的代谢变化.
评估已识别的代谢物的诊断性能.

主要方法:

液体染色学-质谱学 (LC-MS) 用于代谢分析.
分析了30名结肠癌患者的样本.
癌症组织中的代谢物与正常的代谢物进行了比较.

主要成果:

在结肠癌组织中观察到显著的代谢变化.
主要受影响的途径包括酸,TCA循环,糖解,银河糖和丁酸盐代谢.
腺单酸盐 (AMP),脱氧胺单酸盐 (dTMP),果糖和D-葡萄糖的调节失调,并显示诊断AUC>0.7.

结论:

AMP,dTMP,果糖和D-葡萄糖在结肠癌诊断方面表现出色.
这些代谢物代表了结肠癌诊断的有希望的新生物标志物.
对于临床应用,需要进一步验证.

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