异构酶和表皮酶:功能糖的生产中的概述和实际应用
Liqun Jin1,2,3, Qing Wan1,2,3, Shuiping Ouyang1,2,3
1The National and Local Joint Engineering Research Center for Biomanufacturing of Chiral Chemicals, Zhejiang University of Technology, Hangzhou, China.
Critical reviews in food science and nutrition
|October 9, 2023
概括
功能糖的生物合成提供了健康益处,并满足了行业的需求. 蛋白质工程和酶固定改善生物催化剂,克服大规模安全生产的挑战.
科学领域:
- 生物技术和食品科学 生物技术和食品科学
- 功能糖的酶合成功能糖的酶合成
背景情况:
- 功能性糖因其健康益处和工业需求而受到越来越多的关注.
- 酶合成提供了高效,选择性的生产,但面临着诸如酶不稳定性和安全性等挑战.
研究的目的:
- 审查功能糖的酶生物合成的进展.
- 确定克服生产障碍和确保食品安全的战略.
主要方法:
- 蛋白质工程 (定向进化,理性设计) 以提高生物催化剂的性能.
- 酶固定技术可以降低成本并提高工艺效率.
- 用食品级表达系统来确保安全.
主要成果:
- 蛋白质工程和酶不动化在提高酶稳定性和催化效率方面表现有前途.
- 食品级系统解决了功能性糖生产中的安全问题.
- 下游加工 (分离,净化,结晶) 仍然是提高效率和成本效益的关键挑战.
结论:
- 尽管面临当前的挑战,生物催化剂和下游技术的进步正在为工业规模的功能糖生产铺平道路.
- 持续的研究和技术创新对于实现各种行业功能糖的全部潜力至关重要.
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