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尼阿普塔默 (Ni aptamer):DNA模仿他的标签以识别Ni-NTA

Raunak Jahan¹, Achut Prasad Silwal¹, Siddhartha Kalpa Samadhi Thennakoon¹

¹Department of Chemistry and Center for Photochemical Sciences, Bowling Green State University, Bowling Green, Ohio 43403, USA. tanx@bgsu.edu.

Chemical communications (Cambridge, England)

|October 9, 2023

概括

尼阿普特是第一个识别Ni-NTA的aptamer,它像His标签一样用于净化. 这种核酸工具具有强烈的结合性,可以用伊米达或EDTA解.

科学领域:

生物化学生物化学
分子生物学分子生物学
生物技术是生物技术.

背景情况:

他的标签和Ni-NTA广泛用于蛋白质净化.
以核酸为基础的替代His-tag可以提供新的净化策略.

研究的目的:

介绍和描述Ni Apt,一个用于Ni-NTA识别的新型aptamer.
评估Ni Apt作为净化和识别应用中的分子工具的潜力.

主要方法:

与Ni-NTA结合的阿巴胺结合亲和力的表征.
使用伊米达和EDTA评估化条件.

主要成果:

Ni Apt 是第一个具有 Ni-NTA 的特征解离常数的亚胺.
Ni Apt对Ni-NTA表现出强大的结合亲和力 (Kd = 106 nM).
Ni Apt可以从Ni-NTA树脂中用伊米达或EDTA出.

结论:

Ni Apt作为核酸类似于His-tag用于基于Ni-NTA的净化.
Ni Apt是用于核酸净化和识别的多功能分子工具.

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