相关实验视频

Updated: Jul 14, 2025

Techniques for the Evolution of Robust Pentose-fermenting Yeast for Bioconversion of Lignocellulose to Ethanol

Techniques for the Evolution of Robust Pentose-fermenting Yeast for Bioconversion of Lignocellulose to Ethanol

Published on: October 24, 2016

通过肠道酸原 Blautia schinkii 从糖醇中产生乙醇

Raphael Trischler¹, A Poehlein², R Daniel²

¹Molecular Microbiology & Bioenergetics, Institute of Molecular Biosciences, Johann Wolfgang Goethe University, Frankfurt, Germany.

Environmental microbiology

|October 9, 2023

概括

蓝菌将糖醇代谢成酸盐和乙醇,这对肠道健康和生物乙醇生产至关重要. 这项研究揭示了能使甘油分解的关键酶,扩大了我们对肠道微生物功能的理解.

科学领域:

微生物学微生物学
代谢工程是代谢工程.
肠道微生物组研究研究

背景情况:

人的肠道微生物群在健康和疾病中起着双重作用,受细菌发酵产品的影响.
虽然人们越来越了解肠道微生物组的组成,但其功能代谢活动仍未得到充分探索.
布劳蒂亚属因其与人类福祉的关联而得到认可,但其特定的代谢能力在很大程度上未被描述.

研究的目的:

为了研究 Blautia schinkii 的代谢潜力,特别是它对糖的利用.
确定参与B. schinkii. glycerol代谢的酶和途径.
评估B. schinkii的糖代谢对人类健康和工业应用的影响.

主要方法:

用甘油作为唯一的碳来源种植B. schinkii.
发酵最终产品 (乙酸盐,乙醇) 的分析.
转录组分析和生物化学测试以确定关键酶 (糖醇脱酶,二氧酸激酶).

主要成果:

B. schinkii利用糖醇,产生乙酸盐和乙醇.
鉴定糖脱酶和二氧酸激酶,将糖与糖解联系起来.
B. schinkii可以适应高度的甘油 (高达1.5M),产生乙醇.
在二氧化上表现出增长,证实其进入糖解.

结论:

B. schinkii 具有一种用于糖代谢的功能性途径,将其转化为乙酸和乙醇.
这些已识别的酶对于将甘油醇导入中央甘油解路径至关重要.
了解B. schinkii的糖醇代谢,可以了解宿主微生物相互作用,并从废物糖醇中产生生物基乙醇的潜力.

更多相关视频

Production of Chemicals by Klebsiella pneumoniae Using Bamboo Hydrolysate as Feedstock

Production of Chemicals by Klebsiella pneumoniae Using Bamboo Hydrolysate as Feedstock

Published on: June 29, 2017

Extraction of Lignin with High β-O-4 Content by Mild Ethanol Extraction and Its Effect on the Depolymerization Yield

Extraction of Lignin with High β-O-4 Content by Mild Ethanol Extraction and Its Effect on the Depolymerization Yield

Published on: January 7, 2019

相关概念视频

Green Algae

Green Algae

Green algae, also referred to as chlorophytes, are different from red algae in having the chloroplasts containing chlorophylls a and b, which give them their distinct green hue. However, they lack phycobiliproteins, preventing them from developing the red or blue-green pigmentation seen in red algae. In terms of photosynthetic pigment composition, green algae closely resemble plants and share a close evolutionary relationship with them. Taxonomically Green algae belong to Phylum Chlorophyta in...

Lipid Catabolism

Lipid Catabolism

Triglycerides serve as crucial long-term energy storage molecules in microorganisms, providing a dense source of metabolic energy. Their breakdown is mediated by lipases, which hydrolyze triglycerides into glycerol and free fatty acids. Each of these components follows distinct metabolic pathways, ultimately contributing to ATP synthesis and cellular energy homeostasis.Glycerol MetabolismGlycerol, released from triglyceride hydrolysis, is phosphorylated by glycerol kinase to form...

Fates of Pyruvate

Fates of Pyruvate

Pyruvate is the end product of glycolysis, where glucose is oxidized to pyruvate, simultaneously reducing NAD+ to NADH. Two molecules of ATP are also produced by substrate-level phosphorylation.
In aerobic organisms, pyruvate is metabolized via the citric acid cycle to produce reduced coenzymes NADH and FADH2. These coenzymes are then oxidized in the electron transport chain to produce ATP and, in the process, regenerate the NAD+ and FAD. As seen in some cell types and organisms, fermentation...

Biosynthesis of Lipids

Biosynthesis of Lipids

Microbial membranes exhibit remarkable diversity in lipid composition, reflecting evolutionary adaptations to various environmental conditions. The three domains of life—Bacteria, Archaea, and Eukarya—synthesize membrane lipids through distinct biosynthetic pathways, leading to fundamental structural differences that impact membrane stability, function, and adaptability.Fatty Acid-Based Lipids in Bacteria and EukaryaBacteria and eukaryotes share a common fatty acid biosynthesis...

Glycolysis: Preparatory Phase

Glycolysis: Preparatory Phase

In cellular metabolism (the complete breakdown of glucose to extract energy), glycolysis is the first step. Glycolysis takes place in the cytoplasm of both prokaryotic and eukaryotic cells. Glucose enters heterotrophic cells in two ways. One method is through secondary active transport, where the transport takes place against the glucose concentration gradient. The other mechanism uses a group of integral proteins called GLUT proteins, also known as glucose transporter proteins. These...

Glycolysis

Glycolysis

Glycolysis, the Embden-Meyerhof pathway, is a central metabolic pathway involved in glucose catabolism. It is highly conserved across most organisms, reflecting its fundamental role in cellular energy production. This process occurs in the cytoplasm and can function both in the presence and absence of oxygen, making it versatile for various organisms and environmental conditions.Stages of GlycolysisGlycolysis is a ten-step pathway that converts glucose into pyruvate, generating a net gain of...