在荷尔斯坦基因组中识别了富含同胞性区域
1Russian Research Institute of Farm Animal Genetics and Breeding - Branch of the L.K. Ernst Federal Science Center for Animal Husbandry, St. Petersburg, Pushkin, Russia.
Vavilovskii zhurnal genetiki i selektsii
|October 9, 2023
概括
荷尔斯坦牛的同卵性 (ROH) 运行被用两种方法确定. 连续运行被证明更准确地检测这些乳制品动物的ROH和内生系数.
科学领域:
- 动物遗传学动物遗传学
- 基因组学就是基因组学.
- 乳牛养殖 乳牛养殖 乳牛养殖
背景情况:
- 同卵性运行 (ROH) 是基因组的部分,其中一个人对所有类型的SNP都是同卵性.
- ROH可以用来推断过去的近亲繁殖,并确定正在选择的区域.
- 了解ROH模式对于管理牲畜种群的遗传多样性至关重要.
研究的目的:
- 使用Illumina BovineSNP50阵列,对荷尔斯坦牛进行基因造型.
- 用连续和滑动运行方法识别和比较同卵性运行 (ROH).
- 评估缺失的基因型调用和异合体SNP值对ROH识别的影响.
主要方法:
- 使用Illumina BovineSNP50阵列对371头荷尔斯坦牛和26头荷尔斯坦公牛进行基因型鉴定.
- 使用 detectRUNS 和 Plink 软件识别 ROH,使用连续和滑动运行方法.
- 分析ROH段长度,染色体分布,并计算内生系数.
主要成果:
- 连续运行发现ROH的平均数量较高 (95.4±2.7在奶牛中) 与滑动运行 (86.0±2.6在奶牛中) 相比.
- 荷尔斯坦公牛的ROH (58.9 ± 1.9) 比牛少 (58.9 ± 1.9).
- 大多数ROH段的长度为1-2 Mb,染色体BTA 14,BTA 16和BTA 7显示最高的ROH覆盖率.
- 牛的平均内生系数为0.111 ± 0.003 (连续) 和0.104 ± 0.004 (滑动),公牛的平均系数为0.078 ± 0.005.
结论:
- 连续运行识别方法在荷尔斯坦牛的ROH检测中更准确.
- 允许的异合性SNP的数量显著影响ROH数据.
- 研究的霍尔斯坦种群的内生繁殖水平与北美的内生繁殖水平相当.
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