基于STR位元多态化的Bos grunniens和Bos taurus的区分
K B Chekirov1, Zh T Isakova2, V N Kipen3
1Kyrgyz-Turkish Manas University, Bishkek, Kyrgyz Republic.
Vavilovskii zhurnal genetiki i selektsii
|October 9, 2023
概括
使用5个微卫星位点进行的分子遗传分析准确地区分了家养的雅克 (Bos grunniens) 和牛 (Bos taurus). 这种快速测试有助于吉尔吉斯斯坦的育种计划,用于雅克的遗传纯度和杂交鉴定.
科学领域:
- 动物遗传学动物遗传学
- 分子生物学分子生物学
- 保护遗传学 保护遗传学
背景情况:
- 区分像雅克 (Bos grunniens) 和牛 (Bos taurus) 等密切相关的物种对于畜牧养殖,杂交发展和保持遗传纯度至关重要.
- 短串重复 (STR) 位点多态性分析为物种识别和遗传资源管理提供了分子遗传方法.
- 精确的差异化对于有效的育种计划和防止农场动物的遗传侵入至关重要.
研究的目的:
- 通过分子遗传分析,评估15个微卫星位点的潜力,以区分家养 (B. grunniens) 和三种牛品种 (B. taurus).
- 为了确定特定的STR位点,在B. grunniens和B. taurus之间具有很高的区分能力.
- 开发一种具有成本效益和准确的分子测试,用于在繁殖计划中识别物种.
主要方法:
- 在15个微卫星位置进行雅克和牛样本的基因定型,包括12个标准ISAG标记物.
- 使用GenAlEx,Structure,PAST和POPHELPER等软件进行统计分析,以评估人口结构和差异化.
- 计算FST值,以确定单个STR位置的差异化潜力.
主要成果:
- 在将样本分配给物种时的高精度:B. taurus的99.6±0.4%,B. grunniens的99.2±2.6%,使用15个STR位点.
- 识别了五个具有最大FST值的STR位点 (BM1818,BM1824,BM2113,CSSM66,ILSTS006),显示出最大的差异化潜力.
- 仅使用这五个选择的STR位置,实现了高分类准确度 (98.8 ± 3.4%的B. grunniens, 99.1 ± 1.2%的B. taurus).
结论:
- 一个由5个STR位点组成的小组在区分家用和牛方面表现出高效率.
- 开发的分子遗传方法为物种识别提供了准确和快速的方法.
- 这种方法适合在吉尔吉斯斯坦的B. grunniens.繁殖和繁殖计划中作为快速测试来实施.
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