从美国获得的Streptococcus suis分离物的种群结构和遗传多样性
Tracy L Nicholson1, Anwar A Kalalah2,3, Mark Eppinger2,3
1National Animal Disease Center, Agricultural Research Service, United States Department of Agriculture, Ames, IA, United States.
Frontiers in microbiology
|October 9, 2023
概括
猪病原体Streptococcus suis在美国表现出广泛的遗传多样性. 全基因组测序显示,分离物不是在地理上聚集,而是按血清型分组,发现了流行抗菌素耐药性基因.
科学领域:
- 微生物学 微生物学
- 基因组学就是基因组学.
- 兽医科学 兽医科学 兽医科学
背景情况:
- 在全球猪业中,Streptococcus suis造成了重大的经济损失和动物福利问题.
- 之前对S. suis的基因组研究主要使用来自美国以外的分离物.
研究的目的:
- 用全基因组测序和cgMLST分析来自美国的S. suis分离物种的种群结构和遗传关系.
- 了解基因组多样性,并识别US S. suis分离物中的关键遗传元素.
主要方法:
- 全基因组测序 (WGS) 在S. suis的分离物上进行.
- 核心基因组多部位序列类型化 (cgMLST) 用于高分辨率的基因组学分析.
- 计算平均核酸标识 (ANI) 来评估基因组相关性.
主要成果:
- 观察到广泛的基因组变异,美国和非美国的分离物间隔在基因组.
- 隔离物并没有按美国的地理位置聚集,而是按血清型分组.
- 发现了普遍存在的抗菌素耐药性基因 (ble, tetO, ermB) 和与病毒性相关的基因 (epf, mrp, sly).
结论:
- 这项研究为美国S. suis分离物建立了高分辨率的基因组学框架,揭示了显著的遗传多样性.
- 血清型是集群的更强的决定因素,而不是S. suis孤立的地理来源.
- 调查结果为生物监测和了解S. suis在美国的流行病学提供了关键数据.
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