作为对铁限制的反应,改变的运动性由尿路病原性E.在尿路病原性E.在尿路病原性E.受lpdA的调节. 大肠杆菌 CFT073
bioRxiv : the preprint server for biology
|October 9, 2023
概括
铁的限制通过lpdA.通过调节鞭毛体表达来增强尿路致病菌大肠杆菌 (UPEC) 运动性. 这一发现对于了解尿路感染病原和开发针对抗生素耐药菌株的新疗法至关重要.
科学领域:
- 微生物学和免疫学
- 细菌病原体的产生
- 分子生物学分子生物学
背景情况:
- 尿路感染 (UTI) 是常见的,尿病原性大肠杆菌 (UPEC) 是主要原因.
- 细菌的运动性和铁的获取是UPEC的关键毒性因素,但它们的相互作用仍未得到充分研究.
- 主体环境的特点是缺铁,这对细菌的生存和毒性构成了挑战.
研究的目的:
- 在UPEC中调查铁限制和运动调节之间的联系.
- 确定在UPEC中控制铁介导运动的特定调节机制.
主要方法:
- UPEC菌株CFT073是在铁有限的条件下使用铁化剂进行培养的.
- 测量了旗基因 (fliC) 促进活性,转录水平,蛋白质表达和表面鞭毛.
- 构建了八个主调节者的突变,专注于由crp,arcA和fis调节的基因.
主要成果:
- 在UPEC中,铁限制显著增加了fliC促进体活性和鞭毛体表达.
- 这种铁调节的运动反应取决于调节器crp,arcA和fis.
- 在小鼠尿路感染模型中,lpdA突变体表现出铁调节运动率的最显著失调和体能降低.
结论:
- 宿主环境中的铁稀缺性通过涉及lpdA的机制积极调节UPEC运动性.
- 这一发现突出了铁代谢和细菌运动性之间的新联系,影响了UPEC的病原性.
- 了解这种调节网络对于开发有效治疗尿路感染至关重要,特别是那些由抗生素耐药的UPEC引起的疗法.
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