综合单细胞多基因分析显示,肌肉纤维类型的基因调节电路是由耐力运动调节的
Aliza B Rubenstein1, Gregory R Smith1, Zidong Zhang1,2
1Department of Neurology, Icahn School of Medicine at Mount Sinai (ISMMS), New York, NY 10029, USA.
bioRxiv : the preprint server for biology
|October 9, 2023
概括
剧烈的耐力运动会在细胞水平上改变人类的骨肌肉. 单核多原子测序揭示了肌肉纤维类型和其他细胞的明显适应,影响基因调节和代谢重塑.
科学领域:
- 运动生理学 运动生理学
- 分子生物学分子生物学
- 基因组学就是基因组学.
背景情况:
- 耐力运动显著影响骨肌肉的健康和功能.
- 了解细胞适应运动对于优化训练和恢复至关重要.
- 之前的研究缺乏单细胞分辨率来绘制复杂的分子反应.
研究的目的:
- 为了研究细胞类型特定的分子适应在人类骨肌肉的急性耐力运动后.
- 识别参与运动诱导改造的监管程序和转录因子网络.
- 为了提供单细胞分辨率洞察力,了解骨肌肉对身体炼的反应.
主要方法:
- 单核 (sn) 多组测序 (RNA-seq 和 ATAC-seq) 在人类巨大的横向肌肉活检上进行.
- 在40分钟耐力运动 (70%的VO2max) 之前和之后收集样本.
- 通过单细胞电路分析,分析了来自14种细胞类型的37,154个细胞核的数据.
主要成果:
- 在肌肉纤维类型中确定了共享和纤维类型特定的监管程序.
- 在快速,缓慢和中等肌肉纤维以及表达Lumican的纤维基原始细胞 (FAP) 中发现了不同的适应.
- 发现了328个转录因子的网络,它们在改变的可访问性网站上起作用,以调节2025个基因.
结论:
- 急性耐力运动会诱导人类骨肌肉中复杂的,细胞类型特定的分子适应.
- 单细胞多基因组测序为运动介导的基因调节和组织重塑提供了前所未有的洞察力.
- 这些发现提升了我们对运动效益和骨肌肉适应的分子基础的理解.
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