热冲击因子1形成核凝聚物,并在激活目标基因之前重组酵母基因组
Linda S Rubio1, Suman Mohajan1, David S Gross1
1Department of Biochemistry and Molecular Biology, Louisiana State University Health Sciences Center, Shreveport, LA 71130.
bioRxiv : the preprint server for biology
|October 9, 2023
概括
热冲击因子 (Hsf1) 迅速重置热冲击反应 (HSR) 基因,并在乙醇压力下在酵母中形成凝结物. 基因激活发生晚些时候,与热应激相比,显示出不同的动力学.
科学领域:
- 分子生物学分子生物学
- 基因组学就是基因组学.
- 细胞生物学 细胞生物学
背景情况:
- 3D基因组拓与昆虫和哺乳动物的转录状态有关.
- 基因组拓与其他真核生物的转录之间的关系尚不清楚.
- 热冲击反应 (HSR) 基因由热冲击因子 (Hsf1) 调节.
研究的目的:
- 调查3D基因组拓在*Saccharomyces cerevisiae*压力下调节基因表达中的作用.
- 为了确定HSF1依赖的基因组重组和凝结物形成是否先于HSR基因的转录激活.
- 为了比较HSR基因对乙醇和热应激反应的动力学.
主要方法:
- 结合近距离测试用于评估基因间相互作用.
- 光显微镜可视化Hsf1凝聚物和基因组定位.
- 对Pol II占用,染色体重塑和RNA表达的分析.
主要成果:
- 在急性乙醇压力下,HSR基因迅速重新定位并形成HSf1依赖的基因间相互作用.
- 含有Hsf1的凝聚物迅速形成,但持续时间比基因间相互作用更长.
- 在乙醇压力下,转录激活,Pol II占用和染色质重塑发生显著晚 (>1小时),与热应激 (2.5-10分钟) 的快速,短暂的反应不同.
结论:
- 作为对蛋白质毒性压力 (乙醇和热量) 的反应,hsf1协调基因组重组和凝结物形成.
- 乙醇压力会诱导一种独特的动态反应,在这种情况下,基因组重新定位和凝聚物形成会先于转录激活.
- 不同的动力学突出了不同压力条件下的HSR基因的独特调节机制.
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