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相关概念视频

Ribosome Profiling02:24

Ribosome Profiling

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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
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RNA-seq03:21

RNA-seq

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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
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相关实验视频

Updated: Jul 14, 2025

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations
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HybriSeq:基于探针的无设备单细胞RNA分析

Daniel Foyt1, David Brown2, Shuqin Zhou2

  • 1UCSF-UC Berkeley Joint Graduate Program in Bioengineering, University of California San Francisco, San Francisco, California, 94143, United States of America.

bioRxiv : the preprint server for biology
|October 9, 2023
PubMed
概括

我们开发了HybriSeq,这是一种使用in situ杂交和测序的敏感单细胞RNA分析方法. 该技术特征细胞异质性,并检测拼接变异和错误注释的转录.

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科学领域:

  • 分子生物学分子生物学
  • 基因组学就是基因组学.
  • 生物技术是生物技术.

背景情况:

  • 单细胞RNA分析对于理解细胞异质性至关重要.
  • 现有的方法可能面临着针对性转录分析的敏感性和范围的限制.

研究的目的:

  • 引入和验证HybriSeq方法用于高灵敏度单细胞RNA分析.
  • 为了证明HybriSeq在分析差异拼接和识别错误注释的转录中的能力.

主要方法:

  • HybriSeq结合了多个探测器的现场混合化与分池条形码.
  • 对条形码探针的测序分析使得在单细胞水平上有针对性的转录分析成为可能.

主要成果:

  • 通过使用多个探针,HybriSeq实现了高灵敏度的RNA检测.
  • 该方法成功地描述了差异拼接事件.
  • 在95个与细胞周期相关的基因中,细胞对细胞的异质性得到了特征.
  • 错误注释的副本被有效地检测出来.

结论:

  • HybriSeq提供了一种灵敏和多功能的方法,用于向单细胞RNA分析.
  • 这种方法增强了细胞异质性和转录完整性的表征.