相关实验视频

Updated: Jul 26, 2026

Visualizing Genetic Variants, Short Targets, and Point Mutations in the Morphological Tissue Context with an RNA In Situ Hybridization Assay

Visualizing Genetic Variants, Short Targets, and Point Mutations in the Morphological Tissue Context with an RNA In Situ Hybridization Assay

Published on: August 14, 2018

检测人类表皮生长因子受体2 (HER2) 放大:一种替代方法的概念证明

Shikha Mudgal¹, Pranoy Paul¹, Bina Ravi²

¹Pathology and Laboratory Medicine, All India Institute of Medical Sciences, Rishikesh, Rishikesh, IND.

|October 9, 2023

概括

作为免疫组织化学标记物,GRB7和PGAP3蛋白表达显示出有前途,用于预测乳腺癌中人类表皮生长因子受体2 (HER2) 放大. 结合GRB7细胞质染色和PGAP3膜性染色表明了对HER2状态的强有力的预测能力.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
遗传学是一个遗传学.

背景情况:

人类表皮生长因子受体2 (HER2) 放大是乳腺癌的关键驱动因素.
17号染色体上的GRB7和PGAP3等基因的共同放大发生在HER2.
确定HER2放大可靠的生物标志物对于向治疗至关重要.

研究的目的:

研究GRB7和PGAP3蛋白产品作为免疫组织化学 (IHC) 标记物的潜力,以检测乳腺癌中的HER2放大.
评估GRB7和PGAP3表达模式对HER2状态的预测值.

主要方法:

分析了135个原发性乳腺癌组织段.
对HER2,GRB7和PGAP3进行了免疫组织化学染色.
评估了GRB7和PGAP3染色 (膜体和细胞质),用于模两可的HER2病例使用光 in situ杂交.

主要成果:

GRB7和PGAP3 IHC染色显示了对HER2状态的中度至强度预测能力.
对于不同的染色模式,个体AUC值在0.754到0.868之间.
结合GRB7细胞质染色和PGAP3膜性染色,AUC达到0.905,表明高预测准确度.

结论:

特别是在组合下,GRB7和PGAP3蛋白表达是乳腺癌中HER2状态的强烈预测因素.
这些标记物有可能作为替代IHC标记物用于HER2放大.
利用共同放大基因的蛋白质产品的策略为技术验证提供了一个有希望的方法.

关键词:

乳腺癌,乳腺癌.乳腺新生体的发生.这是Erbb2bb2 在 grb7 中,grb7 是什么?免疫组织化学免疫组织化学这是一个pgap3gap3gap.

更多相关视频

Author Spotlight: FISH as a Tool for Precise Gene Amplification Assessment in Cancer Specimens

Author Spotlight: FISH as a Tool for Precise Gene Amplification Assessment in Cancer Specimens

Published on: July 12, 2024

Validated Immunochemical Assay for Comprehensive Determination of the Human Epidermal Growth Factor Receptor 2 Released from and Bound to Cells

Validated Immunochemical Assay for Comprehensive Determination of the Human Epidermal Growth Factor Receptor 2 Released from and Bound to Cells

Published on: May 9, 2025

相关概念视频

Mitogens and the Cell Cycle

Mitogens and the Cell Cycle

Mitogens and their receptors play a crucial role in controlling the progression of the cell cycle. However, the loss of mitogenic control over cell division leads to tumor formation. Therefore, mitogens and mitogen receptors play an important role in cancer research. For instance, the epidermal growth factor (EGF) - a type of mitogen and its transmembrane receptor (EGFR), decides the fate of the cell's proliferation. When EGF binds to EGFR, a member of the ErbB family of tyrosine kinase...