齐申玉台药丸对子宫内膜上皮质反应的调控作用 in vitro 在免疫学微环境中
Xiaoli Chen1, Yanxin Xie1, Lin Li1
1Reproductive Medical Center, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, China.
Heliyon
|October 9, 2023
概括
齐申玉泰药 (ZYP) 可能通过调节子宫微环境中的基因表达来增强子宫内膜受体性. 这种传统中医药制剂显示出改善胚胎植入至关重要的因素的潜力.
科学领域:
- 生殖生物学 生殖生物学
- 传统中国医药 传统中国医药
- 细胞生物学 细胞生物学
背景情况:
- 紫玉台药 (ZYP) 是一种在妇女健康中广泛使用的传统中医药 (TCM).
- 它对子宫内膜上皮细胞和受体性的特定影响仍然不完全理解.
- 了解ZYP的影响对于优化其在生殖医学中的使用至关重要.
研究的目的:
- 在模拟的子宫微环境中研究ZYP对子宫内膜上皮细胞的影响.
- 阐明ZYP影响子宫内膜受体性的分子机制.
- 识别受ZYP治疗影响的关键基因和通路.
主要方法:
- 从孕妇中分离子宫自然杀手 (uNK) 细胞,以创建uNK分泌介质,模仿子宫微环境.
- 用含有ZYP的血清和uNK介质治疗石川子宫内膜上皮细胞.
- 使用微阵列分析,定量实时PCR (qRT-PCR) 和ICAM-1的西部涂抹 (WB) 进行基因表达概况.
主要成果:
- 与uNK组相比,ZYP显著改变了石川细胞中的1117个基因的表达.
- 包括CSF1,CSF2,SPP1和ICAM1在内的关键基因在ZYP治疗后显示出显著的上调 (P <0.05).
- 西方斑点分析证实了细胞间细胞粘附分子-1 (ICAM-1) 表达的上调.
结论:
- 在uNK细胞介质的存在下,ZYP可以通过上调ICAM1,CSF1,CSF2,TNF和SPP1.1等关键基因来增强子宫内膜上皮反应.
- 这些发现表明,ZYP有可能改善子宫内膜接受度.
- 需要进一步的体内研究来验证这些机制,并确认ZYP的体内疗效.
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