通过调节NAMPT介导的NAD生物合成,TRAF3IP2驱动介酶干细胞衰老

通过调节NAMPT介导的NAD生物合成,TRAF3IP2驱动介酶干细胞衰老

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Xiaoran Huang¹, Baojuan Liu¹, Yaowen Liang^1,2

¹Department of Emergency Medicine, Guangdong Provincial People's Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangzhou, Guangdong, China.

Heliyon

|October 9, 2023

概括

与TNF受体相关的3因子相互作用蛋白2 (TRAF3IP2) 通过AMPK通路降低NAMPT和NAD+水平,加速中细胞干细胞 (MSC) 衰老. 这为再生医学中衰老的MSCs的复苏提供了新的策略.

科学领域:

细胞生物学细胞生物学
干细胞研究干细胞研究
再生医学是一种再生医学.

背景情况:

中干细胞 (MSC) 中的细胞衰老阻碍了它们在再生医学中的应用.
与TNF受体相关的3因子相互作用蛋白2 (TRAF3IP2) 是一种促炎蛋白,在MSC衰老中没有明确的作用.

研究的目的:

研究TRAF3IP2在调节MSC衰老中的作用.
为了阐明TRAF3IP2诱导的MSC衰老的潜在机制.

主要方法:

使用与衰老相关的β-galactosidase (SA-β-gal) 染色来评估衰老.
量化了TRAF3IP2表达,衰老标志物,尼古丁胺氨酸二核酸 (NAD+) 水平和NAMPT表达.
采用了西式涂抹和NAD+水平测量.

主要成果:

与年轻的MSC (YMSC) 相比,老年MSC (AMSC) 的TRAF3IP2表达率较高.
过度表达TRAF3IP2加速了YMSC衰老;它的降低调节挽救了衰老.
AMSCs显示NAMPT蛋白和NAD+水平降低,与抑制的AMPK信号传递有关.
通过AMPK抑制,TRAF3IP2通过降低NAMPT和NAD+的调节诱导衰老.

结论:

TRAF3IP2通过AMPK通路抑制NAMPT介导的NAD+生物合成,从而加速MSC衰老.
针对TRAF3IP2或激活AMPK/NAMPT提供了一种新的方法来复兴老化的MSC.

关键词:

介质细胞干细胞介质细胞干细胞它们是NAD+NAD+NAD.这个名字叫做NAMPT.衰老是一种衰老.在TRA3IP2中使用TRA3IP2.

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