相关实验视频

Updated: Jul 14, 2025

Isolation of Normal and Cancer-associated Fibroblasts from Fresh Tissues by Fluorescence Activated Cell Sorting FACS

Isolation of Normal and Cancer-associated Fibroblasts from Fresh Tissues by Fluorescence Activated Cell Sorting FACS

Published on: January 14, 2013

乳腺癌中正常和癌症相关纤维细胞之间的生物学差异

Dengdi Hu¹, Wenying Zhuo^1,2,3,4, Peirong Gong¹

¹Affiliated Cixi Hospital, Wenzhou Medical University, Ningbo, 315300, Zhejiang, China.

|October 9, 2023

概括

癌症相关纤维细胞 (CAFs) 比正常纤维细胞 (NFs) 更恶性,并促进乳腺癌的进展. 在CAF中差异表达的microRNA因亚型而异,但它们的基因途径保持一致.

科学领域:

在瘤学瘤学.
细胞生物学细胞生物学
分子生物学分子生物学

背景情况:

癌症相关纤维细胞 (CAF) 是瘤微环境 (TME) 的关键组成部分.
它们在乳腺癌进展中的作用和潜在机制需要进一步阐明.
了解CAF概况对于向癌症治疗至关重要.

研究的目的:

为了在乳腺癌中区分CAFs和正常纤维细胞 (NFs) 之间的生物特征.
研究CAFs促进癌症进展的分子机制.
分析CAF和NF的微RNA (miRNA) 资料.

主要方法:

从乳腺癌患者中分离和描述CAF和NF.
评估形态和生物属性 (生长,运动,入侵).
共同培养系统评估CAF对乳腺癌细胞和miRNA测序的影响.

主要成果:

与NF相比,CAF显示出明显更高的生长率,运动性和促进乳腺癌细胞恶性病变.
RNA测序揭示了CAF中差异表达的miRNA,具有患者特异性的变异性.
对miRNA点基因的丰富分析显示,在不同患者个人资料中,各个功能途径 (GO术语,KEGG途径) 是一致的.

结论:

与NF相比,CAF具有更大的恶性并影响乳腺癌细胞生长,迁移,入侵和化学抵抗.
虽然CAF中的特定miRNA表达因乳腺癌亚型而异,但这些miRNA所准的功能途径被保留.
这些发现凸显了CAF及其miRNA配置文件在乳腺癌中的复杂作用.

关键词:

生物特征生物特征生物特征乳腺癌乳腺癌乳腺癌与癌症相关的纤维细胞.正常的纤维细胞.这是一个微型RNA.

更多相关视频

Isolation of Primary Cancer-Associated Fibroblasts from a Syngeneic Murine Model of Breast Cancer for the Study of Targeted Nanoparticles

Isolation of Primary Cancer-Associated Fibroblasts from a Syngeneic Murine Model of Breast Cancer for the Study of Targeted Nanoparticles

Published on: May 14, 2021

A Mouse Model to Investigate the Role of Cancer-Associated Fibroblasts in Tumor Growth

A Mouse Model to Investigate the Role of Cancer-Associated Fibroblasts in Tumor Growth

Published on: December 22, 2020

相关概念视频

Introduction to Fibroblasts

Introduction to Fibroblasts

Rudolph Virchow discovered spindle-shaped cells called fibroblasts in 1858. Inactive fibroblasts, called fibrocytes, become activated by various stimuli, such as growth factors and inflammatory cytokines. Activated fibroblasts play a crucial role in wound healing, inflammation, formation of new blood vessels, and cancer progression. Uncontrolled activation of fibroblasts results in fibrosis, the excess deposition of fibrous tissue, which can lead to scarring and affect normal organs. This...

The Tumor Microenvironment

The Tumor Microenvironment

Every normal cell or tissue is embedded in a complex local environment called stroma, consisting of different cell types, a basal membrane, and blood vessels. As normal cells mutate and develop into cancer cells, their local environment also changes to allow cancer progression. The tumor microenvironment (TME) consists of a complex cellular matrix of stromal cells and the developing tumor. The cross-talk between cancer cells and surrounding stromal cells is critical to disrupt normal tissue...

Adaptive Mechanisms in Cancer Cells

Adaptive Mechanisms in Cancer Cells

Cancer cells accumulate genetic changes at an abnormally rapid rate due to the defects in the DNA repair mechanisms. From an evolutionary perspective, such genetic instability is advantageous for cancer development. Mutant cell lines accumulate a series of beneficial mutations that contribute to their progression into cancer.
Some of the advantages that cancer cells have on normal cells include - enhanced ability to divide without terminally differentiating, induce new blood vessel formation,...