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YAP1和多组蛋白SCML2之间的不和相互作用决定了细胞命运
Ava M Boston1, Abdulrahman M Dwead1, Marwah M Al-Mathkour1
1Department of Biological Sciences, Center for Cancer Research and Therapeutic Development, Clark Atlanta University, Atlanta, GA, USA.
iScience
|October 9, 2023
概括
波利康布蛋白SCML2和YAP1共因子相互作用,并由雄激素调节. 它们的合作影响细胞生长和瘤生物学,揭示了类固醇激素信号传递的新机制.
科学领域:
- 分子生物学分子生物学
- 细胞生物学 细胞生物学
- 内分泌学 在内分泌学.
背景情况:
- 多组蛋白SCML2和转录辅因子YAP1是细胞过程的关键调节者.
- 它们的分子和功能相互作用在很大程度上仍然没有被描述.
研究的目的:
- 为了研究SCML2和YAP1.1.之间的相互作用.
- 确定雄激素在调节这种相互作用中的作用.
- 阐明SCML2-YAP1相互作用在细胞生长和瘤生物学中的功能后果.
主要方法:
- 免疫学测定和质谱检测用于检测SCML2-YAP1相互作用.
- 近距离结合试验和GST拉下试验用于物理相互作用的确认.
- RNA干扰 (RNAi) 沉默SCML2并评估对雄激素信号传递和细胞生长的影响.
主要成果:
- SCML2与YAP1.1进行物理相互作用.
- SCML2 和 YAP1 之间的相互作用是由人类瘤细胞中的类固醇激素雄激素调节的.
- 沉默SCML2会改变细胞对雄激素信号的反应,影响细胞生长.
- 有证据表明,SCML2和YAP1之间的相互作用是由不同的染色质修饰和转录程序协调的.
结论:
- SCML2和YAP1合作调节细胞生长,生存和瘤生物学.
- 这种合作是类固醇激素信号的下游,特别是雄激素.
- 这些发现揭示了一个涉及SCML2和YAP1在激素驱动的细胞过程中的新机制.
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