德罗沙的氨基末端区域对于微处理器的功能起着至关重要的作用
Amit Prabhakar1, Song Hu2, Jin Tang2
1Cardiovascular Research Institute, University of California, San Francisco, San Francisco, CA 94143, USA.
iScience
|October 9, 2023
概括
德罗莎-NTR对于微RNA的产生和蛋白质合成的依赖营养的控制至关重要. 它的缺失导致全球microRNA枯竭和不受管制的细胞生长.
科学领域:
- 分子生物学分子生物学
- 遗传学 遗传学 是一个
- 细胞生物学 细胞生物学
背景情况:
- 德罗沙是微RNA处理中的关键酶,调节核糖体蛋白的基因表达.
- 德罗莎的氨基末端区域 (Drosha-NTR) 的突变与遗传性出血性远程连接有关,但其功能尚不清楚.
研究的目的:
- 研究Drosha-NTR在微RNA生物发生和细胞调节中的精确功能.
- 阐明Drosha-NTR在依赖营养素的转化控制中的作用.
主要方法:
- 通过删除第5个外显子,产生了一个缺乏氨基末端区域 (ΔN) 的Drosha突变.
- 在野生类型和 ΔN 细胞中评估了 pri-miRNA 处理,microRNA 水平和蛋白质合成.
- 研究了阿尔戈诺特2在primiR-183/96/182加工中的作用.
主要成果:
- 德罗莎 ΔN 突变体在 pri-miRNA 处理方面存在缺陷,导致全球的微RNA 耗尽,除了 miR-183/96/182 集群.
- 阿尔戈纳特2促进了Drosha ΔN细胞中primiR-183/96/182的处理.
- 与野生类型的Drosha不同,ΔN在血清饥饿过程中抵抗降解,导致不受控制的核糖体蛋白质生物发生和蛋白质合成,从而防止生长停止.
结论:
- 德罗莎-NTR对于高效的微RNA生产至关重要.
- 德罗莎-NTR在依赖营养的转化控制和细胞循环调节中起着至关重要的作用.
- 这些发现揭示了与Drosha突变相关的血管疾病背后的分子机制.
相关概念视频
Directing Proteins to the Rough Endoplasmic Reticulum
7.3K
The organelle-specific signaling sequences direct proteins synthesized in the cytosol to their final destination like ER, mitochondria, peroxisomes, etc. Some of the proteins directed to ER are then trafficked via vesicles to other organelles within the cell or the extracellular environment through the Golgi complex. For example, the rough ER synthesizes soluble proteins for transportation to the lysosomes or secretion out of the cell. It can also synthesize transmembrane proteins that can...
7.3K
Conservation of Protein Domains Over Different Proteins
10.9K
Protein domains are small structurally independent units that are part of a single amino acid chain. Although these domains are often structurally independent, they may rely on synergistic effects to perform their functions as part of a larger protein. Protein domains may be conserved within the same organism, as well as across different organisms.
A limited set of protein domains often duplicate and recombine during evolution. These domains can be organized in different combinations to...
A limited set of protein domains often duplicate and recombine during evolution. These domains can be organized in different combinations to...
10.9K
Nucleic Acid Structure
6.2K
The pentose sugar in DNA is deoxyribose, while in RNA the pentose sugar is ribose. The difference between the sugars is the presence of the hydroxyl group on the ribose's second carbon and a hydrogen on the deoxyribose's second carbon. The phosphate residue attaches to the hydroxyl group of the 5′ carbon of one sugar and the hydroxyl group of the 3′ carbon of the sugar of the next nucleotide, which forms a 5′ to 3′ phosphodiester linkage.
DNA Structure
DNA...
DNA Structure
DNA...
6.2K
piRNA - Piwi-interacting RNAs
6.9K
PIWI-interacting RNAs, or piRNAs, are the most abundant short non-coding RNAs. More than 20,000 genes have been found in humans that code for piRNAs while only 2000 genes have been found for miRNAs. piRNAs can act at the transcriptional and post-transcriptional levels and have a vital role in silencing transposable elements present in germ cells. They are also involved in epigenetic silencing and activation. Previously, they were thought to function only in germ cells but new evidence suggests...
6.9K
pre-mRNA Processing
53.0K
In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl...
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl...
53.0K
Ribosomal RNA Synthesis
13.2K
Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
13.2K


