参与泡细胞形成和动脉样硬化发展的枢纽基因的识别和验证通过生物信息学
Da Teng1,2, Hongping Chen3, Wenjuan Jia1,2
1Yantai Yuhuangding Hospital Affiliated to Qingdao University, Yantai, China.
PeerJ
|October 9, 2023
概括
这项研究确定CSF1R和PLAUR是泡细胞发育中的关键基因,为动脉样硬化提供了新的治疗点. 这些发现澄清了泡细胞对动脉样硬化进展的贡献机制.
科学领域:
- 心血管生物学 心血管生物学
- 分子医学是分子医学.
- 基因组学就是基因组学.
背景情况:
- 泡细胞是动脉样硬化病原体的核心,但它们的精确机制仍然不完全理解.
- 鉴定与泡细胞相关动脉样硬化的新生物标志物和治疗点至关重要.
研究的目的:
- 为了确定新的泡细胞生物标志物和动脉样硬化的干预点.
- 阐明泡细胞导致动脉样硬化进展的机制.
主要方法:
- 动脉样硬化和泡细胞数据集的差异基因表达分析来自基因表达综合 (GEO) 数据库.
- 使用R软件 (LIMMA包),KEGG路径分析,GO注释和蛋白质-蛋白质相互作用网络分析 (Cytoscape) 来识别枢纽基因.
- 使用GSE41571数据集,细胞检测和组织样本验证了关键基因 (CSF1R,PLAUR).
主要成果:
- 在动脉样硬化中确定了407个差异表达基因 (DEG),在泡细胞中确定了219个DEG.
- CSF1R和PLAUR作为常见的枢纽基因出现,在独立的数据集和实验模型中得到了显著的验证.
- 在细胞和组织实验中,CSF1R和PLAUR的表达与脂质积累和疾病进展正相关.
结论:
- CSF1R和PLAUR被确定为泡细胞中的关键枢纽基因,在动脉样硬化中发挥着重要作用.
- 这些发现提高了对动脉样硬化机制的理解,并提出了潜在的治疗策略.
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