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在STAYGREEN的自然变化有助于黄瓜的低温耐受性

Shaoyun Dong¹, Caixia Li¹, Haojie Tian¹

¹State Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.

Journal of integrative plant biology

|October 9, 2023

概括

黄瓜的低温应激耐受性是由一个特定的基因变异 (CsSGR) 增强的. 这一发现为培育更坚固的黄瓜品种提供了途径,提高了作物对寒冷和其他环境挑战的抵抗力.

科学领域:

植物科学植物科学
分子生物学分子生物学
遗传学遗传学是一个

背景情况:

低温 (LT) 压力对全球黄瓜产量产生重大影响.
黄瓜中LT耐受性的分子基础尚不清楚.

研究的目的:

为了确定导致黄瓜对LT耐受性的遗传因素.
阐明LT耐受性的分子机制及其改善作物的潜力.

主要方法:

全基因组关联研究 (GWAS) 以确定与LT耐受性相关的单核酸多态 (SNP).
在CRISPR/Cas9基因编辑中创建CsSGR淘汰突变.
对叶绿素含量,活性氧物种 (ROS) 积累和基因表达的分析.

主要成果:

在STAYGREEN (CsSGR) 基因中的特定SNP与LT耐受性密切相关.
CsSGR淘汰突变体表现出增强的LT耐受性,具有增加的叶绿素和减少的ROS.
在CsSGR基因中,对LT敏感的单体型中介于叶绿素降解,而耐受性单体型则没有.
此外,CsSGR突变体对盐度,干旱和菌的耐受性也得到改善.

结论:

CsSGR基因在黄瓜对LT压力的反应中起着至关重要的作用.
一种特定的CsSGR单元型 (HapG) 赋予LT耐受性,并已在高度黄瓜种群中进行选择.
CsSGR 是一个有前途的目标基因,用于培育具有宽频应激耐受性,包括耐寒,耐盐和耐干旱的子.

关键词:

这就是CRISPR/Cas9的作用.在 CsSGRGR.在GWAS中,GWAS就是GWAS.叶绿素的含量叶绿素的含量黄瓜黄瓜的使用方法对低温的耐受性较高.

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