使用轨道升三杂质质谱仪进行深层蛋白质化合物分析,使用并联质量标签和实时搜索
Steven R Shuken1, Graeme C McAlister2, William D Barshop2
1Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, Massachusetts 02115, United States.
Analytical chemistry
|October 9, 2023
概括
新的Orbitrap Ascend质谱仪通过提高灵敏度和速度,为准确的药物效应分析增强了高通量蛋白质组学. 这种先进的仪器能够以更高的定量精度识别更多的蛋白质,包括低丰富的蛋白质.
科学领域:
- 蛋白质组学是指蛋白质组学.
- 质谱测量质量谱测量
- 药物发现 药物发现 药物发现
背景情况:
- 协同质量标签 (TMT) 和三杂质谱仪对于高通量蛋白质组学至关重要.
- 目前对灵敏度和速度的限制阻碍了药物研究的深度蛋白质基因分析.
- Orbitrap Ascend质谱仪是为了克服这些挑战而开发的.
研究的目的:
- 为了评估新的Orbitrap Ascend质谱仪的性能,用于深度蛋白质分子分析.
- 评估其在高精度和速度量化药物诱导蛋白质组变化的能力.
- 为了将其性能与现有的Orbitrap Eclipse系统进行比较.
主要方法:
- 使用Orbitrap Ascend质谱仪进行蛋白质组分析.
- 用于定量测量的同时使用的质量标签 (TMT).
- 分析了分成样本和用药物治疗的癌细胞 (HCT116) 与PIN1抑制剂.
主要成果:
- 与Eclipse相比,Ascend表现出更高的灵敏度和更高的信号噪声比率,特别是在短时间的注射时间.
- 鉴定和量化了更多的和蛋白质,包括低丰富的和蛋白质,并提高了定量精度.
- 在较短的仪器时间内量化了超过8000种蛋白质,并检测了Eclipse错过的治疗相关蛋白质变化.
结论:
- 轨道升显著推进高通量蛋白质组学,提供增强的灵敏度,速度和定量准确性.
- 它允许更深入的蛋白质基因分析和更精确的药物效应特征,特别是在低丰富的蛋白质.
- 这项技术有助于更好地了解药物向相互作用和结构-活性关系,有助于癌症药物开发.
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