细菌细胞外电子转移元件是自旋选择性的
Christina M Niman1, Nir Sukenik1, Tram Dang2
1Department of Physics and Astronomy, University of Southern California, Los Angeles, California 90089, USA.
The Journal of chemical physics
|October 9, 2023
概括
奇拉诱导的自旋选择性 (CISS) 效应促进了金属减少细菌中的电子运输. 这项研究显示了MtrA和STC蛋白质的自旋选择性,影响了整个电子运输通路.
科学领域:
- 微生物学 微生物学
- 生物物理学的生物物理.
- 电化学 电化学 电化学
背景情况:
- 减少金属的细菌使用细胞外电子运输 (EET) 进行呼吸.
- 多基因细胞染色体是EET的关键,跨越细胞膜.
- 在此之前,在细胞表面蛋白质中观察到了性诱导的旋转选择性 (CISS) 效应.
研究的目的:
- 在上游ETE组件中调查CISS:MtrA和STC.
- 为了确定旋转选择性是否存在于膜相关和周等离子体细胞染色体中.
- 探索CISS在完整的细胞外电子运输通路中的作用.
主要方法:
- 使用导电探头原子力显微镜 (cp-AFM).
- 在铁磁基板上吸附的测量蛋白质单层.
- 量化了MtrA和STC的旋转极化.
主要成果:
- 在MtrA和STC中证明了自旋选择性电子传输.
- 确定了MtrA旋转极化在~77%和STC在~35%.
- 观察到在血红蛋白中旋转选择性的潜在长度依赖关系.
结论:
- 旋转依赖相互作用是整个细胞外电子运输通路的组成部分.
- 在降解金属的细菌中,CISS在有效的电子转移中发挥着重要作用.
- 这些发现有助于进一步了解生物能量学和电子转移机制.
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