生物膜抗微生物敏感性测试:我们在哪里,我们可能会去哪里?
1Laboratory of Pharmaceutical Microbiology, Ghent University, Ghent, Belgium.
Clinical microbiology reviews
|October 9, 2023
概括
尽管生物膜生物学的进步,临床实践落后. 需要进行标准化生物膜抗微生物敏感性测试 (AST),以解决生物膜和感染部位抗生素有效性降低的问题.
科学领域:
- 微生物学 微生物学
- 传染性疾病 传染性疾病
- 临床诊断 临床诊断 临床诊断
背景情况:
- 在了解生物膜生物学和降低抗菌素敏感性方面取得了重大进展.
- 临床实践尚未整合这些知识,特别是在抗菌素敏感性测试 (AST) 中.
- 目前的AST方法主要使用浮游生物细胞,忽视生物膜特异性耐药性和微环境影响.
研究的目的:
- 审查生物膜AST的当前状况.
- 为了确定基于生物膜的抗微生物敏感性测试的知识差距.
- 讨论潜在的改进和临床实施的挑战.
主要方法:
- 关于生物膜生物学和抗菌素敏感性测试的文献综述.
- 对当前临床微生物学实践的分析.
- 讨论新兴的生物膜AST方法.
主要成果:
- 对生物膜抗性机制的了解有所增长,但临床转化是有限的.
- 缺乏标准化的生物膜AST方法,阻碍了准确的敏感性确定.
- 感染微环境显著影响微生物生理学和抗生素反应,但在当前的AST中模型不佳.
结论:
- 在临床微生物学中,对标准化生物膜AST方法的需求非常大.
- 弥合研究和临床实践之间的差距需要解决当前的瓶问题.
- 实施基于生物膜的AST对于改善生物膜相关感染的治疗结果至关重要.
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