使用第一空的尿液接种HPV疫苗后的幽默免疫反应的随访:一个纵向队列研究
Laura Téblick1, Jade Pattyn1, Severien Van Keer1
1Centre for the Evaluation of Vaccination (CEV), Vaccine & Infectious Disease Institute (VAXINFECTIO), Faculty of Medicine and Health Sciences, University of Antwerp, Antwerp, Belgium.
Journal of medical virology
|October 9, 2023
概括
第一次空洞尿液显示为检测人类乳头瘤病毒 (HPV) 疫苗反应的非侵入性方法具有前途. 这项研究在尿液和血液样本中发现了可比的抗体水平,支持其在HPV疫苗试验中的使用.
科学领域:
- 免疫学 免疫学 免疫学
- 疫苗学 疫苗学 疫苗学
- 泌尿器科 泌尿器科 泌尿器科 泌尿器科
背景情况:
- 评估对人类乳头瘤病毒 (HPV) 疫苗接种的幽默免疫反应通常需要进行侵入性血液采样.
- 需要使用非侵入性方法来监测疫苗的有效性和免疫记忆.
研究的目的:
- 评估第一空尿作为检测HPV特定抗体的非侵入性替代样本的可用性.
- 为了比较HPV疫苗接种后的配对尿液和血清样本中的抗体度.
主要方法:
- 一项纵向队列研究,涉及58名用9价值HPV疫苗 (9vHPV) 接种疫苗的妇女.
- 在接种疫苗前,接种疫苗后1个月,接种疫苗后3年内,采集了第一次空气尿液和血清样本.
- 类型特定的HPV抗体反应使用类似病毒的基于粒子的IgG多重酶链接免疫吸收试验进行了分析.
主要成果:
- 在接种疫苗后1个月的第一空尿液 (95%-100%) 和血清 (100%) 中发现了可检测的HPV特异性抗体的高百分比.
- 可检测的抗体水平在接种疫苗后的3年内在84%-100%的尿样和98%-100%的血清样本中持续存在.
- 在所有时间点在配对的尿液和血清样本中观察到HPV特异性抗体度之间的显著相关性.
结论:
- 第一次空白尿是HPV疫苗试验中免疫监测的可行的非侵入性样本.
- 基于尿的抗体检测为血液采样提供了一个可行的替代方案,用于评估HPV疫苗反应.
- 这种方法可以简化样本采集,并改善参与者对疫苗研究的遵守.
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