低氧酶3 (LOX3) 表达增强了储存期间玉米内核托科菲罗尔的保留
Shalma Maman1, Vignesh Muthusamy2, Ashvinkumar Katral1
1Division of Genetics, ICAR-Indian Agricultural Research Institute, New Delhi, India.
Molecular biology reports
|October 9, 2023
概括
富含维生素E的玉米在储存过程中会失去多科菲醇. 在HKI323-PVE玉米中,较低的脂氧酶活性和LOX3基因表达与较高的托科菲醇保留相关,用于生物强化计划.
科学领域:
- 农业科学 农业科学
- 生物化学 生化学
- 遗传学 是一个遗传学.
背景情况:
- 维生素E 缺乏导致神经和与年龄有关的问题.
- 具有有利vte4等位基的玉米杂交物富含α-托科法醇 (维生素E).
- 储存期间的托科菲罗尔降解限制了富含维生素E的玉米的有效性.
研究的目的:
- 研究氧化酶和LOX3基因在储存期间在玉米中的多科菲罗尔降解中的作用.
- 为了比较两个对比的玉米杂交品种中的多菲醇保留.
主要方法:
- 在两个玉米杂交品种 (HKI323-PVE和HKI193-1-PVE) 中,在六个月内评估了多菲罗尔降解.
- 测量了每月间隔的脂氧酶活性和LOX3基因表达.
- 分析了托科菲罗尔水平,脂氧酶活性和LOX3表达之间的相关性.
主要成果:
- 在两种杂交品种中都发生了显著的托卡法醇降解,其中HKI193-1-PVE的保留率较低.
- 与HKI193-1-PVE相比,HKI323-PVE表现出较低的脂氧酶活性和LOX3基因表达.
- 氧酶活性和LOX3表达在储存后120天达到峰值,与α-托科菲罗尔水平负相关.
结论:
- HKI323-PVE 由于低的脂氧酶活性和LOX3表达,显示出高的托科菲醇保留,使其对维生素E生物强化有价值.
- 这项研究强调了LOX3基因在储存中的生物强化玉米中维持α-托科菲罗尔水平的关键作用.
- 蛋白质-蛋白质关联网络分析表明vte4和LOX基因的独立作用.
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