重新发明代谢途径:在开花植物中,佐诺酸的独立演变
Matilde Florean1, Katrin Luck1, Benke Hong1
1Department of Natural Product Biosynthesis, Max Planck Institute for Chemical Ecology, Jena 07745, Germany.
概括
氧胺 (BXD) 是一种具有抗微生物特性的植物化合物. 这项研究发现了藻中新的BXD通路,揭示了它们使用不同的酶多次独立进化.
科学领域:
- 植物生物化学 植物生物化学
- 代谢学 代谢学 代谢学
- 进化生物学是进化的生物学.
背景情况:
- 氧类药物 (BXDs) 是一种具有抗微生物和抗养性质的特殊植物代谢物.
- 在远距离相关的植物序列中发现了BXDs,这表明它们的生物合成的独立进化.
- 虽然在草中已知BXD通路,但它们在藻中的生物合成仍然没有特征.
研究的目的:
- 确定和描述两种与远距离相关的鱼物种, *Aphelandra squarrosa* 和 *Lamium galeobdolon* 的氧化 (BXD) 生合成途径.
- 为了研究BXD生物合成的进化起源和酶机制在.
主要方法:
- 代谢和转录组分析被用来指导途径的发现.
- 在 *Nicotiana benthamiana* 中进行了途径复制,以验证已识别的酶.
- 进行了对BXD通路的比较分析,其中包括花和单花 (草).
主要成果:
- 在*A. squarrosa*和*L. galeobdolon*中确定和描述了BXD生物合成途径.
- 欧迪科特通路利用含有黄素的双重功能单氧酶,与草中发现的两个单独的细胞染色体P450s不同.
- 进化上不相关的酶,包括细胞染色体P450s,2-oxoglutarate依赖的二氧化原酶,UDP-glucosyltransferase和甲基转移酶,都被招募用于BXD合成.
结论:
- 这项研究报告了在鱼中发现BXD生物合成途径的发现.
- 这些发现表明,BXD在植物王国中至少发生了三次独立进化.
- 多样化的酶机制突出显示了BXD合成中的显著代谢可塑性,在类似的化学逻辑中使用不同的酶.
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