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热电纳米流体检测单细胞内部的热异质性
Hao Ding1, Kang Liu1, Xinlu Zhao1
1State Key Laboratory of Analytical Chemistry for Life Science, Chemistry and Biomedicine Innovation Center (ChemBIC), School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, Jiangsu, China.
Journal of the American Chemical Society
|October 9, 2023
概括
一种新的纳米管电热仪 (NET) 能够以高灵敏度实时测量细胞内温度. 这种突破性的工具揭示了免疫治疗期间癌细胞的热异质性,
科学领域:
- 生物物理
- 细胞生物学
- 纳米技术
背景情况:
- 准确的细胞内温度测量对于了解细胞功能和调节至关重要.
- 现有的方法缺乏实时单细胞分析所需的灵敏度和时空分辨率.
研究的目的:
- 开发一种新型的纳米管电温度计 (NET),用于准确的实时细胞内温度监测.
- 研究单个细胞内的热力学,包括对刺激和免疫治疗的反应.
主要方法:
- 基于温度控制的离子迁移和热电反应的纳米管电热计 (NET) 的开发.
- 使用静电配置进行敏感电压测量.
- 实现高时空分辨率 (100 nm,0.9 ms) 和热分辨率 (25 mK).
主要成果:
- 网络显示了高度敏感的热电反应 (高达11.1mV K-1),明显超过以前的方法.
- 成功监测稳定状态细胞的热波动和药物管理后的热量变化.
- 免疫治疗期间单个癌细胞的热异质性首次报告,与生存和耐药性相关联.
结论:
- NET提供了一种可靠且高度灵敏的微观温度监测方法.
- 揭示了癌症细胞在免疫治疗期间的热行为,可能解释免疫逃避和治疗抵抗机制.
相关概念视频
Specific Heat
The specific heat capacity of a substance refers to the energy required to increase the temperature of one gram of that substance by one degree Celcius. Specific heat capacity is often represented in calories (cal), grams (g), and degrees Celsius (oC), but can also be expressed in joules (J), kilograms (kg), and Kelvin (K), among other units.
For example, increasing the temperature of one gram of water by 1°C requires one calorie of heat energy and can be written as 1 cal/g-°C, or 4186 J/kg/K.
For example, increasing the temperature of one gram of water by 1°C requires one calorie of heat energy and can be written as 1 cal/g-°C, or 4186 J/kg/K.
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Near absolute zero temperatures, in the presence of a magnetic field, the majority of nuclei prefer the lower energy spin-up state to the higher energy spin-down state. As temperatures increase, the energy from thermal collisions distributes the spins more equally between the two states. The Boltzmann distribution equation gives the ratio of the number of spins predicted in the spin −½ (N−) and spin +½ (N+) states.
Heat Flow and Specific Heat
Heat is a type of energy transfer that is caused by a temperature difference, and it can change the temperature of an object. Since heat is a form of energy, its SI unit is the joule (J). Another common unit of energy often used for heat is the calorie (cal), which is defined as the energy needed to change the temperature of 1 g of water by 1 °C, specifically between 14.5 °C and 15.5 °C, since the energy needed shows a slight temperature dependence. Another commonly used unit is the kilocalorie...

