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在接受植入前遗传测试的患者中,高精子DNA碎片化增加了胚胎无体积率

Wanting Fu¹, Qiuying Cui¹, Ziyao Yang¹

¹Centre for Reproductive Medicine, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China; Henan Key Laboratory of Reproduction and Genetics, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China; Henan Provincial Obstetrical and Gynaecological Diseases (Reproductive Medicine) Clinical Research Centre, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China; Henan Engineering Laboratory of Preimplantation Genetic Diagnosis and Screening, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China.

Reproductive biomedicine online

|October 9, 2023

概括

高精子DNA碎片化 (SDF) 与经过细胞内精子注射 (ICSI) - 植入前遗传检测 (PGT) 的患者胚胎积率的增加有关. 然而,高SDF没有影响转移euploid胚胎后的结果.

科学领域:

生殖医学生殖医学
遗传学遗传学是一个
胚胎学胚胎学

背景情况:

精子DNA碎片化 (SDF) 是影响男性生育能力和生殖结果的潜在因素.
内细胞质精子注射 (ICSI) 与植入前遗传检测 (PGT) 结合使用,以提高辅助生殖的成功率.
在PGT周期中,高SDF和胚胎形形成之间的关系需要进一步澄清.

研究的目的:

在接受ICSI-PGT的夫妇中,研究高精子DNA碎片化 (SDF) 和胚胎形率之间的关联.
确定SDF水平是否影响胚胎染色体异常的可能性.

主要方法:

对426对接受ICSI-PGT的夫妇进行了回顾性研究,他们的心脏类型正常.
使用精子染色体结构试验评估的精子DNA碎片.
倾向性得分匹配 (PSM) 和通用线性混合模型用于分析SDF和体积率之间的关系.

主要成果:

通过ROC曲线分析确定了30%的SDF值.
在PSM (132对夫妇) 之后,高SDF组 (>30%) 显示出显著更高的SDF和胚胎无体积率 (69.36%对53.96%,P <0.001).
>30%的SDF与胚胎形性增加有显著的相关性 (调整后OR为1.70,P=0.049),但没有影响转移后的结果.

结论:

在接受ICSI-PGT的患者中,高的SDF (>30%) 与胚胎无体积率的增加有关.
增加的SDF并没有对胚胎和临床结果产生负面影响,而是在euploid胚胎转移后.
对于正常心型型的患者,SDF评估可能是PGT周期中的一个有价值的辅助.

关键词:

胚胎动积分率是多少胚胎动积分率是多少在细胞体内注射精子.男性因素是男性因素.在植入前进行遗传检测.精子DNA碎片化的情况

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