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从生物流体和自然水中提取精神活性物质的液相微提取,这种微提取是基于天然的深度环氧溶剂

Patricia García-Atienza¹, Héctor Martínez-Pérez-Cejuela¹, José Manuel Herrero-Martínez¹

¹Department of Analytical Chemistry, University of Valencia, Dr Moliner 50, 46100, Burjassot, Valencia, Spain.

|October 9, 2023

概括

天然深溶解剂 (NADES) 是一种可持续的方法,可以通过液相微提取 (LPME) 来从生物样本中提取精神活性物质. 这种方法增强了液体染色学-并联质谱法 (LC-MS/MS) 的检测.

科学领域:

分析化学分析化学
绿色化学绿色化学
法医毒理学法医毒理学

背景情况:

精神活性物质分析的传统方法通常依赖于有机溶剂,造成环境和健康问题.
液相微提取 (LPME) 是一种有价值的样品制备技术,但其对环境的影响需要改进.

研究的目的:

开发和验证一种新的,可持续的方法来从水性和生物基质中分离精神活性物质.
与传统的基于溶剂的方法相比,评估开发的方法的绿色信誉.

主要方法:

在液相微提取 (LPME) 过程中使用了天然的深溶性溶剂 (NADES).
确定了优化的NADES组成 (L-menthol:octanoic acid, 1:2 M比) 和提取参数.
分析使用液体染色学-并联质谱法 (LC-MS/MS) 进行.
方法验证包括使用经过认证的样本进行线性,精度,回收和准确性评估.

主要成果:

基于NADES的LPME达到16倍的预度因子.
证明了出色的线性 (LOQ 0.00060.05μg L-1 到 100μg L-1),精度 (RSD ≤ 8%) 和恢复率 (> 70%).
对经过认证的唾液和血清样本的分析产生了与认证值相比的统计结果.
绿色指标的评估表明,与使用或二甲的LPME相比,绿色指标的可持续性更高.

结论:

拟议的基于NADES的LPME方法对于复杂矩阵中精神活性物质的分析是有效和准确的.
这种方法为传统的基于溶剂的提取技术提供了更绿色和更可持续的替代方案,与环境原则保持一致.
该方法显示出在法医和临床毒理学中常规应用的巨大潜力.

关键词:

深层欧性溶剂深层欧性溶剂滥用药物的药物滥用药物液相微提取液相微提取方法精神活性物质是一种精神活性物质.

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