细菌社区的网络复杂性在不同的土地使用模式下,在肠的微生物群中驱动抗生素耐药性
Fang Luo1, Yang Zhao2, Jia-Yang Xu3
1Key Laboratory of Urban Environment and Health, Ningbo Urban Environment Observation and Research Station, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China; Zhejiang Key Laboratory of Urban Environmental Processes and Pollution Control, CAS Haixi Industrial Technology Innovation Center in Beilun, Ningbo 315830, China; State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Key Laboratory for Pesticide Residue Detection of Ministry of Agriculture and Rural Affairs, Institute of Agro-Product Safety and Nutrition, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China.
抗生素耐药性基因 (ARG) 在土壤中比在肠中更为丰富. 土地利用和pH等环境因素对土壤动物微生物群中的ARG分布产生重大影响.
科学领域:
- 环境微生物学环境微生物学
- 土壤生态学 土壤生态学
- 抗微生物耐药性 抗微生物耐药性
背景情况:
- 土壤生态系统中的抗生素耐药性基因 (ARG) 越来越令人担忧.
- 在各种土地使用类型的土壤动物中对ARG模式的理解有限.
研究的目的:
- 在土壤和虫肠道中调查ARG的丰富性和配置.
- 确定土地利用对ARG分布的影响.
- 确定影响ARG的关键环境因素.
主要方法:
- 从四种土地使用类型收集样本:农田,医院,公园和山区公园.
- 使用高通量定量PCR (HT-qPCR) 来量化162个ARG.
- 对非生物 (如pH) 和生物 (如细菌网络复杂性) 因素的分析.
主要成果:
- 与肠相比,土壤中的ARG丰度和数量更高.
- 虫的肠道表现出一个更加异质的ARG特征.
- 土地使用显著改变了ARG的丰富性,数量和同时发生的模式.
- 土壤pH负面影响ARG配置文件;细菌网络的复杂性对ARG产生积极影响.
结论:
- 土地利用是ARG在土壤和虫微生物组中分布的关键驱动因素.
- 虫在塑造ARG异质性方面发挥着重要作用.
- 像pH和细菌群体结构这样的环境因素是ARG概况的关键决定因素.
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