在Piscirickettsia salmonis感染期间,大西洋鱼头和SHK-1细胞系中的替代拼接:比较的转录组调查
Yeny Leal1, Valentina Valenzuela-Muñoz1, Cristian Gallardo-Escárate1
1Interdisciplinary Center for Aquaculture Research (INCAR), Universidad de Concepción, P.O. Box 160-C, Concepción, 4030000, Chile.
Fish & shellfish immunology
|October 9, 2023
概括
替代拼接 (AS) 在大西洋鱼中发挥着关键作用.
科学领域:
- 水产养殖免疫学 水产养殖免疫学
- 分子生物学分子生物学
- 文字转录学 (Transcriptomics) 是一个学科.
背景情况:
- 在大西洋鱼水产养殖中,Piscirickettsia salmonis会导致大量的死亡.
- 替代拼接 (AS) 越来越多地被认为是其在宿主-病原体相互作用和免疫反应中的作用.
- 在细菌感染期间了解鱼的AS对于疾病管理至关重要.
研究的目的:
- 在Piscirickettsia salmonis感染期间调查大西洋鱼的替代拼接转录组资料.
- 为了比较鱼细胞系 (SHK-1) 和头组织感染后的AS事件.
主要方法:
- SHK-1细胞系和大西洋鱼头脏组织感染了P. salmonis.
- 总RNA被提取并在感染后的0,7和14天接受了Illumina测序.
- 使用RNA-Seq数据分析了差异替代拼接 (DAS) 事件.
主要成果:
- 在SHK-1细胞 (16,830事件) 和头 (13,820事件) 中,DAS事件的峰值发生在感染后7天.
- 替代的第一个外体 (AF) 是最丰富的AS类型,而相互排斥的外体 (MX) 是最不丰富的.
- 在SHK-1细胞中,DAS基因与核酸代谢有关,而在头中,它们与免疫反应有关.
结论:
- 在大西洋鱼感染Piscirickettsia salmonis时,替代拼接发生了显著的变化.
- AS有助于调节宿主对细菌感染的转录反应.
- 这些发现有助于我们更好地理解分子层面的病原体与宿主相互作用.
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