相关实验视频
Updated: Jul 14, 2025

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Isolation and Th17 Differentiation of Naïve CD4 T Lymphocytes
Published on: September 26, 2013
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卡片9/中性粒细胞信号轴促进IL-17A介导的结脊柱炎
Holly L Rosenzweig1,2, Emily E Vance1,2, Kofi Asare-Konadu1
1Molecular Microbiology and Immunology, Oregon Health & Science University, Portland, Oregon, USA.
Annals of the rheumatic diseases
|October 9, 2023
概括
卡德9 (caspase招募域含有蛋白9) 在中性粒细胞功能中起着至关重要的作用,驱动致病性Th17反应和结性脊髓炎 (AS) 的发展. 这项研究揭示了一种新型的中性粒细胞内在机制,它是AS病原体的基础.
科学领域:
- 免疫学 免疫学 免疫学
- 类风湿病学 类风湿病学
- 分子生物学分子生物学
背景情况:
- 在CARD9中的多态性与结性脊髓炎 (AS) 有关.
- 了解CARD9在AS病变发生过程中的细胞机制至关重要.
研究的目的:
- 研究CARD9如何控制致病性Th17反应.
- 阐明CARD9在小鼠模型和人类患者的AS发病中的作用.
主要方法:
- 在SKG小鼠 (野生型,Card9淘汰赛,中性粒细胞枯竭) 和小鼠中性粒细胞和CD4+T细胞的体外共同培养的实验.
- 分析中性粒细胞与T细胞的比率以及AS患者的浴性脊柱炎功能指数.
- 在体外研究中使用异性中性粒细胞:T细胞共同培养来评估CARD9变体和IL-17A生产.
主要成果:
- 在SKG小鼠中,Card9对于Th17细胞诱导,关节炎和脊髓炎至关重要,其功能是Dectin-1的下游.
- 在T细胞中表达Card9对于关节炎是不可或缺的;中性粒细胞是Th17扩张和疾病的关键驱动因素.
- 表达CARD9的中性粒细胞增强了CD4+T细胞的IL-17A产生.
- 在AS患者中观察到类似的机制,其中来自HLA-B27+患者的中性粒细胞扩大了Th17细胞,而CARD9 S12N变体增加了IL-17A.
结论:
- CARD9在促进关节致病的Th17反应方面具有一种新的中性粒细胞内在功能.
- 这一发现提供了关于CARD9特异性机制在脊椎关节炎发病的洞察力.
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