LncRNA MEG8通过miR-485-3p/FBXO45轴改善了帕金森病的神经炎症
Xia Lin1, Taotao Tao1, Xinwei He1
1Department of Neurology, Taizhou Central Hospital (Taizhou University Hospital), No.999, Donghai Avenue, Jiaojiang District, Taizhou, 318000, Zhejiang, China.
Acta neurologica Belgica
|October 9, 2023
概括
长非编码RNA母性表达8 (MEG8) 显示了作为帕金森病 (PD) 诊断生物标志物的潜力. 通过调节miR-485-3p/FBXO45通路,增加的MEG8可能会减少PD中的炎症.
科学领域:
- 神经科学是一个神经科学.
- 分子生物学分子生物学
- 遗传学 遗传学 是一个
背景情况:
- 长非编码RNA母性表达8 (MEG8) 与炎症调节有关.
- 在帕金森病 (PD) 中MEG8的作用和机制仍然未被描述.
- 这项研究调查了MEG8在PD中的临床意义和生物功能.
研究的目的:
- 评估MEG8作为帕金森病 (PD) 的诊断生物标志物.
- 阐明MEG8在PD病变发生过程中的生物功能和潜在机制.
- 探索MEG8在缓解与PD相关的炎症反应方面的潜力.
主要方法:
- 血清和细胞MEG8,miR-485-3p和FBXO45水平使用RT-qPCR在102名PD患者,86名AD患者和80名健康对照中量化.
- 使用LPS诱导的微质BV2细胞的体外模型来研究炎症反应.
- 通过ROC曲线分析评估诊断价值,并使用双露西法酶报告员和RIP测定验证了向关系.
主要成果:
- 在PD患者和用LPS治疗的BV2细胞中,MEG8和FBXO45水平显著降低,而miR-485-3p水平则升高.
- 血清MEG8显示出高灵敏度和特异性,可以将PD患者与健康和AD对照区分开来.
- MEG8缓解了LPS诱导的炎症,这种效果取决于miR-485-3p/FBXO45轴,其中MEG8作为miR-485-3p的海绵,抑制它并促进FBXO45的表达.
结论:
- 升高的MEG8作为帕金森病 (PD) 的潜在诊断生物标志物.
- 通过miR-485-3p/FBXO45通路,MEG8可能在PD中产生抗炎作用.
- 这些发现突出了一个涉及MEG8在PD病理生理学中的新型分子机制.
更多相关视频
相关概念视频
01:24Parkinson's Disease: Treatment
283
Neurodegenerative disorders, such as Parkinson's Disease (PD), involve the gradual and irreversible destruction of neurons in particular brain areas. These disorders exhibit standard features like proteinopathies, selective vulnerability of some neurons, and an interaction of intrinsic properties, genetics, and environmental influences in neural injury.
Parkinson's Disease is primarily a result of the loss of dopaminergic neurons in the substantia nigra pars compacta. The cornerstone of...
Parkinson's Disease is primarily a result of the loss of dopaminergic neurons in the substantia nigra pars compacta. The cornerstone of...
283
01:37Neural Regulation
39.5K
Digestion begins with a cephalic phase that prepares the digestive system to receive food. When our brain processes visual or olfactory information about food, it triggers impulses in the cranial nerves innervating the salivary glands and stomach to prepare for food.
39.5K
01:15Parkinson's Disease: Overview
579
Neurodegenerative disorders are progressive diseases that cause irreversible damage and loss to neurons in specific brain areas. Examples of these disorders include Parkinson's disease, Alzheimer's disease, Multiple Sclerosis (MS), and Amyotrophic Lateral Sclerosis (ALS). These disorders share characteristics such as proteinopathies, selective neuronal vulnerability, and a complex interplay between genetic and environmental factors. The primary therapeutic goal for these conditions is...
579
01:22MicroRNAs
3.0K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.0K
02:39lncRNA - Long Non-coding RNAs
8.6K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
8.6K
02:26NF-κB-dependent Signaling Pathway
7.5K
The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The...
NF-κB-dependent Signaling Mechanism
The...
7.5K