在牙周炎中Wnt信号
Zeliha Güney1,2, Şivge Kurgan3, Canan Önder1
1Faculty of Dentistry Department of Periodontology, Ankara University, 06500-Cankaya, Ankara, Turkey.
Clinical oral investigations
|October 9, 2023
概括
牙周炎扰乱了牙组织中的Wnt/β-catenin信号通路,由Wnt蛋白水平升高和β-catenin局部变化表明. 这种干扰与牙周病的炎症和组织破坏有关.
科学领域:
- 口腔生物学 口腔生物学
- 分子生物学分子生物学
- 免疫学 免疫学 免疫学
背景情况:
- Wnt/β-catenin信号通路对于组织平衡和发育至关重要.
- 牙周炎是一种慢性炎症性疾病,导致牙周组织的破坏.
研究的目的:
- 为了研究Wnt/β-catenin信号通路在牙周炎患者的牙周组织中的活性.
- 为了将Wnt通路的改变与牙周炎的炎症状态和组织破坏相关联.
主要方法:
- 定量PCR (Q-PCR) 用于测量Wnt 3a,Wnt 5a,Wnt 10b和β-catenin的基因表达.
- 免疫组织化学分析以确定β-catenin,Wnt 5a和Wnt 10b蛋白质的地形定位.
- 评估TNF-α对炎症状态和Runx2作为破坏媒介的评估.
主要成果:
- 与对照组相比,牙周炎组织中Wnt 3a,Wnt 5a和Wnt 10b的表达显著更高 (p <0.05).
- 在牙周炎患者的结缔组织中观察到内核β-catenin染色,与对照组中的细胞内染色形成鲜明对比.
- 牙周炎患者连接组织中Wnt5a和Wnt10b蛋白水平升高,特别是在III期C级.
结论:
- 与牙周炎相关的牙周炎症破坏了Wnt/β-catenin信号通路.
- 改变的Wnt信号传递有助于在牙周炎的发病过程中破坏组织恒温和宿主诱导的组织破坏.
- 这些发现突显了Wnt通路调节失调在牙周病期间免疫炎症反应中的作用.
相关概念视频
Canonical Wnt Signaling Pathway
8.8K
The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which...
8.8K
Non-Canonical Wnt Signaling Pathways
7.3K
Wnt is a zygotic effect gene that is expressed during very early embryonic development. It regulates various processes in animals starting from early development through the adult stage, such as organogenesis in the embryo and maintenance of neuronal and blood stem cells. Wnt proteins can induce a wide variety of intracellular pathways depending upon the specific abilities of different Wnt ligands to form a complex with shared and cognate receptors in the presence of different co-receptors. The...
7.3K
Role Of Notch Signalling In Intestinal Stem Cell Renewal
2.1K
Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
2.1K
Notch Signaling Pathway
4.3K
The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not...
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not...
4.3K
TGF - β Signaling Pathway
7.4K
The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...
7.4K


