转录基因分析表明,ABA信号传递和碳供应在花的尾芽的差异生长中是相关的
Zhiwei Luo1,2, Dan Jones3, Sarah Philp-Wright2
1The New Zealand Institute for Plant and Food Research Limited, Auckland, New Zealand.
BMC plant biology
|October 9, 2023
概括
植物芽的分枝是由芽位置和营养物质的可用性调节的. 转录组分析揭示了芽生长与休眠的独特遗传程序,受激素和碳供应的影响.
科学领域:
- 植物生物学 植物生物学
- 遗传学 遗传学是一种遗传学.
- 发展生物学 发展生物学
背景情况:
- 植物发芽分枝表现出塑性,受营养和光线等环境线索的影响.
- 尾芽的生长取决于位置;在花中,基本芽 (1-3) 通常处于休眠状态,而尖端芽 (6-7) 可以生长.
研究的目的:
- 为了研究木中尾芽外生长的遗传调节.
- 了解营养的可用性 (酸盐水平) 如何影响芽基因表达和生长潜力.
主要方法:
- 在不同位置的花尾芽的转录组分析 (RNA-seq).
- 在具有不同酸盐水平的介质中培养植物,以操纵芽生长.
- 同表达分析以确定调节芽生长的候选基因.
主要成果:
- 在生长的 (尖端) 和不生长的 (基底) 芽之间,已经确定了超过5000个差异表达的基因.
- 角芽显示出更高的细胞激素和辅酶基因表达,而基底芽表达了与休眠相关的基因 (strigolactone,abscisic acid).
- 酸盐的可用性显著改变了基因表达,低酸盐转移到高酸盐转移减少了角芽中的ABA相关基因表达.
结论:
- 同一植物的芽之间的发育潜力的差异反映在不同的转录组配置文件中.
- 芽生长在基底芽中被积极抑制,可能是由于有限的碳供应.
- 植物平衡尾芽的生长与资源的可用性,保持一些芽的休眠期以供未来的发展.
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