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相关概念视频

Ribosome Profiling02:24

Ribosome Profiling

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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
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Regulated mRNA Transport02:22

Regulated mRNA Transport

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In eukaryotes, transcription and translation are compartmentalized; an mRNA is first synthesized in the nucleus and then selectively transported to the cytoplasm for protein synthesis. Before transport, a pre-mRNA undergoes several steps of post-transcriptional modifications including splicing, 5' capping, and the addition of a poly-adenine tail. Various proteins bind to the pre-mRNA during these modifications. The mRNA transport takes place with the help of multiple proteins playing...
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Cell Specific Gene Expression01:58

Cell Specific Gene Expression

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Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
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Chromatin Position Affects Gene Expression02:35

Chromatin Position Affects Gene Expression

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Chromatin is the massive complex of DNA and proteins packaged inside the nucleus. The complexity of chromatin folding and how it is packaged inside the nucleus greatly influences  access to genetic information. Generally, the nucleus' periphery is considered transcriptionally repressive, while the cell's interior is considered a transcriptionally active area. 
Topologically Associated Domains (TADs)
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DNA Microarrays

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Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
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相关实验视频

Updated: Jul 14, 2025

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection
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细胞邻居EX:从空间转录组学数据中解读邻居依赖的基因表达.

Hyobin Kim1,2, Amit Kumar3,4,5, Cecilia Lövkvist6

  • 1Department of Computational Biomedicine, Cedars-Sinai Medical Center, Hollywood, CA, USA.

Molecular systems biology
|October 10, 2023
PubMed
概括
此摘要是机器生成的。

这项研究引入了CellNeighborEX,一种分析邻近细胞如何影响空间转录学中的基因表达的新方法. 它揭示了影响发育和癌症转移的细胞与细胞相互作用.

关键词:
细胞与细胞的相互作用.蜂通讯 蜂通讯是指蜂通讯的形式.邻居依赖基因 邻居依赖基因空间转录学 空间转录学

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科学领域:

  • 细胞生物学 细胞生物学
  • 基因组学就是基因组学.
  • 生物信息学是一种生物信息学.

背景情况:

  • 细胞通过连接体,受体和间隙结进行通信,以感知它们的微环境.
  • 现有的方法难以捕捉细胞微环境对基因表达的全部影响.
  • 了解细胞-细胞通信对于生物过程和疾病至关重要.

研究的目的:

  • 开发一种新的方法,CellNeighborEX,用于在空间转录组学数据中研究细胞邻居依赖基因表达.
  • 识别与特定邻近细胞类型相关的基因组.
  • 为了揭示超越连接体-受体信号传递的细胞-细胞相互作用的新见解.

主要方法:

  • CellNeighborEX根据空间转录组学数据中的位置或转录组混合物对细胞进行分类.
  • 该方法分析了相对于邻细胞类型的基因表达模式.
  • 空间可视化技术用于验证.

主要成果:

  • 识别了与各种组织 (老鼠胚胎,大脑,肝癌) 中特定邻近细胞类型相关的多样化的基因组.
  • 观察到邻近的细胞类型影响基因表达,影响发展和转移等关键生物过程.
  • 通过空间可视化验证邻居诱导的基因表达.

结论:

  • 细胞NeighborEX提供了一个强大的工具来探索邻居依赖基因表达在空间转录学.
  • 邻近的细胞类型在调节基因表达方面发挥着重要作用,影响关键的生物功能.
  • 这种方法揭示了潜在的新基因和参与细胞与细胞相互作用的机制.