相关实验视频

Updated: Jul 14, 2025

Fabrication and Characterization of Layer-By-Layer Janus Base Nano-Matrix to Promote Cartilage Regeneration

Fabrication and Characterization of Layer-By-Layer Janus Base Nano-Matrix to Promote Cartilage Regeneration

Published on: July 6, 2022

用于弹性矩阵修复的JNK2沉默脂质纳米粒子

Shataakshi Dahal¹, Suraj Bastola¹, Anand Ramamurthi¹

¹Department of Bioengineering, Lehigh University, Bethlehem, Pennsylvania, USA.

Journal of biomedical materials research. Part A

|October 10, 2023

概括

在腹腔大动脉瘤 (AAA) 模型中,PEGylated脂质纳米颗粒有效地传递JNK siRNA来降低c-Jun N-终端激酶 (JNK2) 和矩阵金属蛋白酶 (MMP),促进弹性质修复.

科学领域:

生物医学工程生物医学工程
分子生物学分子生物学
心血管研究研究心血管研究

背景情况:

腹腔大动脉瘤 (AAA) 的进展涉及JNK2过度表达,导致矩阵金属蛋白酶 (MMP) 介导的弹性矩阵分解.
之前的研究表明,在AAA模型中,JNK2基因倒置促进了弹性素表达,并减少了MMPs.
裸体siRNA在传递,稳定性和潜在毒性方面面临挑战,需要改进的传递系统.

研究的目的:

开发PEGylated脂质纳米粒子 (LNPs) 以有效地传递JNK siRNA.
为了证明成功的JNK2淘汰和下游减弱MMP2表达.
评估对弹性纤维和弹性纤维组装相关基因表达对AAA修复的影响.

主要方法:

使用薄膜水化 (100-200 nm大小,30-40 mV泽塔电位) 制备PEGylated LNP.
将 JNK siRNA 载荷的 LNP 传递给细胞因子激活的老鼠动脉瘤光滑肌细胞 (EaRASMC).
量化JNK2,MMP2,弹性素 (ELN) 和lysyl氧化酶 (LOX) 的基因和蛋白质表达.

主要成果:

带有JNK siRNA的PEGylated LNPs成功地减少了EaRASMC培养物中的JNK2表达.
观察到MMP2基因和蛋白质表达显著下降.
弹性素 (ELN) 和酸氧化酶 (LOX) 表达的上升趋势表明弹性纤维组合得到改善.

结论:

在AAA研究中,阴离子PEGylated LNP是JNK siRNA传递的有效载体.
这种方法增强了JNK2的淘汰和MMP2的衰减,促进了弹性质的恒常性.
临床临床治疗提供了一种有前途的治疗干预策略,可以预防AAA的进展.

关键词:

腹部大动脉动脉瘤腹部大动脉动脉瘤弹性矩阵再生修复复复原修复基因沉默是对基因进行沉默的方法.脂质纳米颗粒的使用方法 siRNA疗法是指一种治疗方法.

更多相关视频

Fabrication of a Biomimetic Nano-Matrix with Janus Base Nanotubes and Fibronectin for Stem Cell Adhesion

Fabrication of a Biomimetic Nano-Matrix with Janus Base Nanotubes and Fibronectin for Stem Cell Adhesion

Published on: May 10, 2020

Long-term Silencing of Intersectin-1s in Mouse Lungs by Repeated Delivery of a Specific siRNA via Cationic Liposomes. Evaluation of Knockdown Effects by Electron Microscopy

Long-term Silencing of Intersectin-1s in Mouse Lungs by Repeated Delivery of a Specific siRNA via Cationic Liposomes. Evaluation of Knockdown Effects by Electron Microscopy

Published on: June 21, 2013

相关概念视频

Elastin is Responsible for Tissue Elasticity

Elastin is Responsible for Tissue Elasticity

Elastic fiber contains the protein elastin along with lesser amounts of other proteins and glycoproteins. The main property of elastin is that it will return to its original shape after being stretched or compressed. Elastic fibers are prominent in elastic tissues found in skin and the elastic ligaments of the vertebral column.
Ligaments and tendons are made of dense regular connective tissue, but in ligaments not all fibers are parallel. Dense regular elastic tissue contains elastin fibers and...