![Dynamic Imaging of Chimeric Antigen Receptor T Cells with [18F]Tetrafluoroborate Positron Emission Tomography/Computed Tomography](/_next/image?url=https%3A%2F%2Fcloudfront.jove.com%2FCDNSource%2Fteasers%2F62334.jpg&w=3840&q=50)
卡特-T疗法向纤维素素阳性固体瘤细胞的额外域B
Jie Tang1, Nan Liu1, Yongjie Zhu1
1Department of Targeting Therapy & Immunology and Laboratory of Animal Tumor Models, Cancer Center and Department of Respiratory and Critical care Medicine and Frontiers Science Center for Disease-related Molecular Network, West China Hospital, Sichuan University, Chengdu, Sichuan, China.
Immunological investigations
|October 10, 2023
概括
研究人员开发了新的化学抗原受体T细胞 (CAR-T) 疗法,针对固体瘤的纤维素蛋白 (EDB-FN) 额外B域. 这些针对EDB-FN的CAR-T细胞证明了有效的瘤细胞杀伤,并具有临床应用的潜力.
科学领域:
- 免疫治疗是一种免疫疗法.
- 在瘤学瘤学.
- 生物技术是生物技术.
背景情况:
- 卡特-T细胞疗法在B细胞恶性瘤中取得了成功,但由于目标限制,在固体瘤中滞后.
- 纤维蛋白B额外域 (EDB-FN) 是一个有前途的癌症特异性标,在固体瘤上升调节,在正常组织中表达最小.
- EDB-FN的特性使其成为开发新型免疫疗法的理想目标.
研究的目的:
- 设计和评估针对癌症特异性抗原EDB-FN.的新型化学抗原受体T细胞 (CAR-Ts).
- 评估EDB-FN向的CAR-T对固体瘤细胞系的疗效和特异性.
- 调查这些新型CAR-T结构的安全概况.
主要方法:
- 通过lentiviral转导使用 (APT0) 和单链抗体 (CGS2) 构建两个针对EDB-FN的CAR-T.
- 在实验室中评估CAR-T细胞毒性,使用 luciferase 试验和通过ELISA释放细胞因子 (IFN-γ).
- 通过光成像和敲击测试评估CAR-T细胞动态和向特异性.
主要成果:
- 成功构建了针对EDB-FN的APT0 CAR-T和CGS2 CAR-T.
- 这两种CAR-T变异都表现出EDB-FN阳性固体瘤细胞系的广泛杀死,伴随着IFN-γ释放.
- 卡尔-T细胞对正常的T细胞或HEK-293T细胞没有表现出毒性,这表明其安全性概况有利.
结论:
- APT0 CAR-T和CGS2 CAR-T代表了针对固体瘤的EDB-FN的新型免疫治疗剂.
- 这些CAR-T细胞在体外有效地识别和消除各种EDB-FN阳性固体瘤细胞.
- 开发的CAR-T疗法显示出未来在固体瘤治疗中的临床应用的巨大潜力.
相关概念视频
02:57Targeted Cancer Therapies
7.7K
The targeted cancer therapies, also known as “molecular targeted therapies,” take advantage of the molecular and genetic differences between the cancer cells and the normal cells. It needs a thorough understanding of the cancer cells to develop drugs that can target specific molecular aspects that drive the growth, progression, and spread of cancer cells without affecting the growth and survival of other normal cells in the body.
There are several types of targeted therapies against...
There are several types of targeted therapies against...
7.7K
01:27Tumor Immunotherapy
539
Immunotherapy is a treatment that boosts or manipulates the immune system to fight diseases, including cancer. For instance, by stimulating an immune response through vaccinations against viruses that cause cancers, like hepatitis B virus and human papillomavirus, these diseases can be prevented. Nonetheless, some cancer cells can avoid the immune system due to their rapid mutation and division. The immune response to many cancers involves three phases: elimination, equilibrium, and escape.
539