抗生素不会直接诱导acrAB的表达,而是通过依赖RAMA的途径
Vito Ricci1, Jaswant Kaur1, Jack Stone1
1Antimicrobials Research Group, Institute of Microbiology and Infection, College of Medical and Dental Science, University of Birmingham , Birmingham, United Kingdom.
Antimicrobial agents and chemotherapy
|October 10, 2023
概括
沙门氏菌 Typhimurium 是一个类型.
科学领域:
- 微生物学 微生物学
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 在Salmonella Typhimurium中,acrAB排泄对于多药性耐药性至关重要.
- 它的诱导主要由转录激活剂调节,如Rama,MarA,SoxS和Rob.
- 了解精确的调节机制和动力学对于开发有效的抗微生物战略至关重要.
研究的目的:
- 调查Rama是否是沙门氏菌Typhimurium中AcrAB表达的唯一诱导剂.
- 探索其他转录激活剂在acrAB诱导中的参与.
- 阐明了acrAB和ramA的诱导中的动力差异.
主要方法:
- 使用的绿色光蛋白 (GFP) 和红色光蛋白 (RFP) 转录记者融合.
- 评估了acrAB,ramA,marA和soxS的基因表达.
- 暴露沙门氏菌Typhimurium各种化合物的亚抑制度.
主要成果:
- 13种化合物通过Rama,MarA,SoxS和Rob依赖的途径诱导了acrAB的表达.
- 没有测试过的抗生素直接诱导了acrAB表达.
- 诱导acrAB的化合物也触发了一般的应激反应.
- acrAB表达是暂时的,而ramA表达显示持续的诱导.
- 拉玛似乎具有更广泛的监管作用,超出了排水调节.
结论:
- 拉玛是主要的,但不是唯一的,在沙门氏菌Typhimurium中诱导acrAB的途径.
- AcrB排泄的抗生素基质不会直接诱导acrAB的表达.
- 亚克拉巴和拉玛诱导的差异动力学表明复杂的调节网络和拉玛的全球作用.
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