相关实验视频

Updated: Jul 14, 2025

Optical Control of a Neuronal Protein Using a Genetically Encoded Unnatural Amino Acid in Neurons

Optical Control of a Neuronal Protein Using a Genetically Encoded Unnatural Amino Acid in Neurons

Published on: March 28, 2016

通过面膜向的非遗传光开关调节机械敏感通道

Matteo Moschetta¹, Vito Vurro¹, Valentina Sesti^1,2

¹Center for Nano Science and Technology, Istituto Italiano di Tecnologia, Via Rubattino, 81, 20134 Milano, Italy.

The journal of physical chemistry. B

|October 10, 2023

概括

机械敏感离子通道,如TRAAK,调节细胞潜能. 使用Ziapin2的新光刺激方法通过膜变形来调节这些通道,为通道病变提供了新的治疗点.

科学领域:

细胞电生理学细胞电生理学
膜生物物理学膜生物物理学
神经生物学神经生物学神经生物学

背景情况:

机械敏感离子通道将机械刺激转化为生化信号,这对于生理过程至关重要.
与TWIK相关的花酸刺激的K+通道 (TRAAK) 维持了静止膜潜力和神经生物学功能.
TRAAK失调与,心律失常和慢性疼痛等疾病有关,使它们成为治疗点.

研究的目的:

研究一种新的非遗传光刺激方法来调节TRAAK离子通道.
探索Ziapin2,一种两性亚博,在控制膜性质和通道活性中的机制.
评估膜变形作为非选择性机械敏感通道调制工具的潜力.

主要方法:

人类TRAAK (hTRAAK) 通道在HEK293T细胞中的表达.
使用Ziapin2,一种两性亚博,用于光感应的膜稀释和放松.
测量K+离子流和细胞膜潜力的变化,以响应Ziapin2和光刺激.

主要成果:

在Ziapin2插入等离子体膜时,会招募hTRAAK,促进K+流出和细胞超极化.
由Ziapin2引起的光诱导的膜放松导致hTRAAK通道的关闭.
这种关闭导致细胞膜的补偿性去极化.

结论:

齐亚平2通过光控制的膜变形有效调节hTRAAK通道活动.
由Ziapin2进行的膜稀释打开了TRAAK通道,而放松则关闭了它们.
这种光刺激策略提供了一种非选择性机械敏感通道调制的非遗传方法,具有治疗意义.

更多相关视频

Whole-cell Patch-clamp Recordings for Electrophysiological Determination of Ion Selectivity in Channelrhodopsins

Whole-cell Patch-clamp Recordings for Electrophysiological Determination of Ion Selectivity in Channelrhodopsins

Published on: May 22, 2017

Use of Label-free Optical Biosensors to Detect Modulation of Potassium Channels by G-protein Coupled Receptors

Use of Label-free Optical Biosensors to Detect Modulation of Potassium Channels by G-protein Coupled Receptors

Published on: February 10, 2014

相关概念视频

Mechanically-gated Ion Channels

Mechanically-gated Ion Channels

Mechanically-gated ion channels are proteins found in eukaryotic and prokaryotic cell membranes that open in response to mechanical stress. Tension, compression, swelling, and shear stress can alter the conformation of the protein, opening a transmembrane channel that allows the passage of ions for signal transmission. In eukaryotes, mechanically-gated channels are distributed in several regions like the neurons, lungs, skin, bladder, and heart, where they play critical roles in numerous...

Channel Rhodopsins

Channel Rhodopsins

Most organisms use photoreceptors to sense and respond to light. Examples of photoreceptors include bacteriorhodopsins and bacteriophytochromes in some bacteria, phytochromes in plants, and rhodopsins in the photoreceptor cells of the vertebral retina. The light-sensitive property of these receptors is because of the bound chromophores, such as bilin in the phytochromes and retinal in the rhodopsins.
Rhodopsins belong to the family of cell surface proteins called G-protein coupled receptors,...

G-Protein Gated Ion Channels

G-Protein Gated Ion Channels

GPCRs are primarily responsible for our sense of smell, taste, and vision. The binding of a sensory stimulus activates GPCR to stimulate effector proteins, many of which are ion channels in the sensory organs. GPCRs modulate the opening and closing of the target ion channels either directly by binding them, or by releasing second messengers that activate these channels. As ions move across the membrane, the membrane potential is altered, which induces an appropriate response.
Sensory...